Course detail
Analytical Chemistry II
FCH-BC_ANC2Acad. year: 2023/2024
This course aims to introductuion students to the basic methods and techniques of instrumental analytical chemistry. Teaching is structured in the following thematic areas: Physical aspects of analytical methods. Analytical signal and its qulitative and quantitative evaluation. Principles of selected instrumental methods-spectrometric and electroanalytical methods, their qualitative and quantitative aspects and practical examples. Potentiometry. Voltametry and polarography (classical, pulse, stripping). Titrations with polarizable electrodes. Electrogavimetry, coulometry. Conductometry, dielectrimetry. Optical methods, their classification, properties of electromagnetic radiation. Refractometry, interferometry, polarimetry, nephelometry, turbidimetry. Electronic absorption spectra, instrumentation. UV-VIS molecular spectrophotometry. Luinescence methods: molecular fluorescence, phosphorescence, chemiluminescence, bioluminescence. Absorption and emission atomic spectrometry. Principles and importance of chromatographic and electromigration methods. Introduction to analytical chemometry. Methods of quantitative evaluation.
Language of instruction
Number of ECTS credits
Mode of study
Guarantor
Entry knowledge
Rules for evaluation and completion of the course
Condition for passing the exam is to obtain credit from the exercise of Analytical Chemistry II.
The exam is oral, evaluate the level of knowledge of theory and practice of analytical chemistry and creative approach to solving analytical problems.
Attendance at lectures is recommended, but not checked. Attendance at seminars is compulsory and is checked.
The students' work is controlled by three partial written tests during the semester in computational exercises.
For students in combined form of study are organized consultations on the topics for full-time students.
Consultations are also provided to full-time students on request.
Aims
By completion of the course the student will gain the following knowledge and skills:
1. They will know in depth the principles of simple instrumental analytical methods and possibilities of their application.
2. They will be able to select the optimal method for solving of specific analytical problems of analytical practice.
3. They will adopt the basic principles of chemometric treatment of analytical results obtained using simple instrumental-analytical methods.
Study aids
Prerequisites and corequisites
Basic literature
K. Záruba a spol.: Analytická chemie 1. a 2. díl, 2016 (CS)
Kotouček M., Skopalová J.: Příklady z analytické chemie. Vydavatelství Univerzity Palackého Olomouc, 1997. (CS)
Recommended reading
Volka K.: Analytická chemie II. VŠCHT Praha, 1997. (CS)
Elearning
Classification of course in study plans
- Programme BKCP_CHCHTE Bachelor's 2 year of study, summer semester, compulsory
- Programme BKCP_AAEFCH Bachelor's 2 year of study, summer semester, compulsory
- Programme BPCP_AAEFCH Bachelor's 2 year of study, summer semester, compulsory
- Programme BPCP_CHCHTE Bachelor's 2 year of study, summer semester, compulsory
- Programme BKCP_CHTM Bachelor's 2 year of study, summer semester, compulsory
- Programme BPCP_CHTM Bachelor's 2 year of study, summer semester, compulsory
- Programme BPCP_CHTOZP Bachelor's 2 year of study, summer semester, compulsory
- Programme BKCP_CHTOZP Bachelor's 2 year of study, summer semester, compulsory
- Programme BPCP_CHTPO Bachelor's
specialization CHPL , 2 year of study, summer semester, compulsory
specialization PCH , 2 year of study, summer semester, compulsory
specialization BT , 2 year of study, summer semester, compulsory - Programme BKCP_CHTPO Bachelor's
specialization PCH , 2 year of study, summer semester, compulsory
specialization BT , 2 year of study, summer semester, compulsory
specialization CHPL , 2 year of study, summer semester, compulsory - Programme BPCP_CHMA Bachelor's 2 year of study, summer semester, compulsory
- Programme BPCP_CHTN Bachelor's 2 year of study, summer semester, compulsory
- Programme CP_CZV_SP Lifelong learning 1 year of study, summer semester, compulsory
Type of course unit
Lecture
Teacher / Lecturer
Syllabus
Topic I: Electroanalytical Methods
2. Potentiometry
3. Voltammetry and polarography (classical, differential pulse, dissolution, adsorption). Potentiometric dissolution analysis, titration with polarizable electrodes. Electrogravimetry, Coulometry, Conductometry.
Topic II: Spectrometry
4. Classification of spectral methods, basic physical principles, properties of electromagnetic radiation. Non-spectral methods: Refractometry, interferometry, polarimetry, nephelometry, turbidimetry. UV-VIS spectrophotometry - principle, properties, device design, application.
5. Luminescent methods: molecular fluorescence, phosphorescence, chemiluminescence, bioluminescence and their analytical use.
6. Mass spectrometry.
7. Fundamentals of optical atomic spectrometry.
Topic III: Separation Methods
Basic theory of chromatographic separation process. Planar Chromatography, Column Chromatography.
9. Liquid chromatography
10. Gas chromatography.
11. Separation in the electric field. Planar techniques (gel electrophoresis), capillary techniques (capillary zone electrophoresis, isotachophoresis, capillary isoelectric focusation).
12. Tandem techniques.
Topic IV: Evaluation of results
13. Basics of analytical chemometry. Procedures for evaluating analytical results and calibration functions.
Exercise
Teacher / Lecturer
Syllabus
Elearning