Course detail

Advanced Molecular Biotechnology

FCH-DCO_MOBAcad. year: 2022/2023

The course is focused on exploatation of molecular biotechnology in construction of genetically modified organisms, particularly plants. Some part is focused on the use of transgenic plants and animals for enhancing the quality of foods and on their use as bioreactors for production of important compounds. Attention is focused on the safety of foods with GMO for men´s health and environment.

Language of instruction

Czech

Mode of study

Not applicable.

Guarantor

prof. Mgr. Václav Brázda, Ph.D.

Department

Institute of Food Science and Biotechnology (ÚCHPBT)

Learning outcomes of the course unit

Theoretical aspects of the use of molecular biotechnology (application of recombinant DNA techniques) in the preparation of useful products with the impact in food production.

Prerequisites

Courses from Biochemistry, Microbiology and Molecular Biotechnology.

Co-requisites

Not applicable.

Planned learning activities and teaching methods

The course uses teaching methods based on individual consultation. The e-learning system (LMS Moodle) is available to teachers and students.

Assesment methods and criteria linked to learning outcomes

Examination is oral.

Course curriculum

1. Synthesis of commercial products by genetically modified microorganisms.
2. Manipulation of gene expression in plants.
3. Transgenic plants resistant to biotic stress.
4. Transgenic plants resistent to abiotic stress.
5. Enhancing of quality of foods of plant origin using transgenic plants.
6. Modification of plant nutritional content (aminoacids, lipids, vitamins, iron, phosphorus).
7. Modification of food plant taste and appearance (coloration, sweetness,starch).
8. Plants as bioreactors (production of antibodies, polymers).
9. Phytoremediation.
10. Transgenic animals and foods.
11. Safety of foods containing transgenic organisms.
12. The impact of genetically modified organisms on the environment.
13. Economic issues.

Work placements

Not applicable.

Aims

Lectures cover the use of transgenic organisms in the preparation of useful products, including foods. The attention is focused on to the foods of plant origin and their safety mainly.

Specification of controlled education, way of implementation and compensation for absences

The course uses teaching lectures and individual consultation which are connected to the PhD thema.

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Glick B.R., Molecular biotechnology, American Society for Microbiology, 2017 (CS)
Rossana M., Genetic Engineering and Biotechnology, Callisto Reference, 2018 (CS)
Snustad P.D, Genetika, MUNI Press, 2017 (CS)