Course detail
Laboratory Classes in Molecular Biotechnology
FCH-MC_MOB_PAcad. year: 2021/2022
The course is focused on practical mastering of method polymerase chain reaction in identification of bacteria of genus Lactobacillus in milk product (probiotic drink). PCR-ready DNA is isolated from the product using magnetic microspheres. PCR mixtures are prepared after estimation of DNA concentration and quality. DNA is amplified in conventional PCR and in real-time PCR. Working out of for protocols in the form of posters is part of the course.
Language of instruction
Number of ECTS credits
Mode of study
Guarantor
Learning outcomes of the course unit
Prerequisites
Co-requisites
Planned learning activities and teaching methods
The teaching requires 26 hours alltogether.
E-learning system LMS Moodle is available for teachers and students.
Assesment methods and criteria linked to learning outcomes
Course curriculum
2. Cultivation of Lactobacillus cells for isolation of bacterial DNA. Estimation of purity of bacterial culture.
Cell lysis and preparing of crude cell lysate. Removing of proteins using phenol extraction. Precipitation of DNA with ethanol. Determination of DNA integrity using agarose gel electrophoresis. Preparation of a protocol in the form of poster.
3. Spectrophotometric estimation of DNA concentration and purity. Preparation of PCR mixture for amplification of specific part of Lactobacillus DNA using purified DNA. Preparation of negative control. PCR amplification. Detection of PCR product using agarose gel electrophoresis. Estimation of length of amplicon with help of DNA standard and programme Bionumerics. Preparation of a protocol in the form of poster.
4. Isolation of whole DNA from crude cell lysates of dairy product (probiotic drink) using magnetic particles P(HEMA-co-GMA). Amplification of DNA by PCR in real time. Preparation of a protocol in the form of poster.
5. Introduction to bioinformatics - lecture a practical excersize on PC.
6. Test and control of protocols.
Work placements
Aims
Specification of controlled education, way of implementation and compensation for absences
Recommended optional programme components
Prerequisites and corequisites
Basic literature
Recommended reading
Elearning
Classification of course in study plans
- Programme NPCP_CHMA Master's
specialization CHBL , 1 year of study, winter semester, compulsory-optional
- Programme NKCP_CHTPO Master's
specialization BCH , 1 year of study, winter semester, compulsory-optional
- Programme NPCP_CHTP Master's
branch NPCO_CHTP , 1 year of study, winter semester, compulsory-optional
- Programme NKCP_CHTP Master's
branch NKCO_CHTP , 1 year of study, winter semester, compulsory-optional
- Programme NPCP_MA Master's
branch NPCO_MA , 1 year of study, winter semester, compulsory-optional
Type of course unit
Guided consultation in combined form of studies
Teacher / Lecturer
Laboratory exercise
Teacher / Lecturer
Elearning