Publication detail
Optimization of polymerase chain reaction for detection of live and dead probiotic lactobacillus cells
TRACHTOVÁ, Š. ŠPANOVÁ, A. RITTICH, B.
Original Title
Optimization of polymerase chain reaction for detection of live and dead probiotic lactobacillus cells
Type
abstract
Language
English
Original Abstract
The general application of ethidium monoazide (EMA) is based on penetration into dead cells with copromised membrane integrity. DNA bound to EMA cannot be PCR amplified. Experiments were performed using probiotic bacterial strein Lactobacillus paracasei subsp. paracasei CCDM 211/06. The lactobacillus species specific primers were used for DNA amplification. The PCR products (250 bp) were separated using electrophoresis in 1.8 % agarose gel. The method was applied for live and dead lactobacillus cells identification in dairy products.
Keywords
ethidium monoazide; dead and live bacterial cells
Authors
TRACHTOVÁ, Š.; ŠPANOVÁ, A.; RITTICH, B.
RIV year
2008
Released
4. 6. 2008
Publisher
Univerzita P.J. Šafárika
Location
Košice, Slovenská Republika
Pages from
55
Pages to
55
Pages count
1
BibTex
@misc{BUT61084,
author="Štěpánka {Trachtová} and Alena {Španová} and Bohuslav {Rittich}",
title="Optimization of polymerase chain reaction for detection of live and dead probiotic lactobacillus cells",
booktitle="Proceedings o International Probiotic Conference, ISSN 1336-863X",
year="2008",
edition="1",
pages="55--55",
publisher="Univerzita P.J. Šafárika",
address="Košice,
Slovenská Republika",
note="abstract"
}