Detail publikace

Optimization of polymerase chain reaction for detection of live and dead probiotic lactobacillus cells

TRACHTOVÁ, Š. ŠPANOVÁ, A. RITTICH, B.

Originální název

Typ

abstrakt

Jazyk

angličtina

Originální abstrakt

The general application of ethidium monoazide (EMA) is based on penetration into dead cells with copromised membrane integrity. DNA bound to EMA cannot be PCR amplified. Experiments were performed using probiotic bacterial strein Lactobacillus paracasei subsp. paracasei CCDM 211/06. The lactobacillus species specific primers were used for DNA amplification. The PCR products (250 bp) were separated using electrophoresis in 1.8 % agarose gel. The method was applied for live and dead lactobacillus cells identification in dairy products.

Klíčová slova

ethidium monoazide; dead and live bacterial cells

Autoři

TRACHTOVÁ, Š.; ŠPANOVÁ, A.; RITTICH, B.

Rok RIV

2008

Vydáno

4. 6. 2008

Nakladatel

Univerzita P.J. Šafárika

Místo

Košice, Slovenská Republika

Strany od

Strany do

Strany počet

BibTex

@misc{BUT61084,
  author="Štěpánka {Trachtová} and Alena {Španová} and Bohuslav {Rittich}",
  title="Optimization of polymerase chain reaction for detection of live and dead probiotic lactobacillus cells",
  booktitle="Proceedings o International Probiotic Conference, ISSN 1336-863X",
  year="2008",
  edition="1",
  pages="55--55",
  publisher="Univerzita P.J. Šafárika",
  address="Košice,
Slovenská Republika",
  note="abstract"
}