Publication detail
DNA isolation by magnetic particles – first step in identification of microorganisms in food matrices
CHROBOKOVÁ, M. RITTICH, B. ŠPANOVÁ, A. HORÁK, D.
Original Title
DNA isolation by magnetic particles – first step in identification of microorganisms in food matrices
Type
conference paper
Language
English
Original Abstract
Isolation of DNA is an important step in the molecular diagnostics of microorganisms. A high quality of isolated DNA is necessary for DNA amplification by the polymerase chain reaction (PCR). Total DNA was successfully isolated from cell lysates using magnetic P(HEMA-co-EDMA) and P(HEMA-co-GMA) microspheres covered by carboxyl groups in the presence of PEG 6000 and sodium chloride. DNA isolation by magnetic particles is comparable to the traditional extraction procedures such as phenol/chloroform one and non-magnetic silica particles but with the added advantages of reduced organic solvent consumption and processing time.
Keywords
magnetic particles, DNA, microorganisms, foods; polymerase chain reaction
Authors
CHROBOKOVÁ, M.; RITTICH, B.; ŠPANOVÁ, A.; HORÁK, D.
RIV year
2013
Released
21. 3. 2013
Publisher
Československá spoločnosť mikrobiologická
Location
Bratislava-Praha
ISBN
978-80-971422-0-9
Book
Čo nového v mikrobiologii
Pages from
94
Pages to
98
Pages count
222
BibTex
@inproceedings{BUT99830,
author="Maria {Chroboková} and Bohuslav {Rittich} and Alena {Španová} and Daniel {Horák}",
title="DNA isolation by magnetic particles – first step in identification of microorganisms in food matrices",
booktitle="Čo nového v mikrobiologii",
year="2013",
pages="94--98",
publisher="Československá spoločnosť mikrobiologická",
address="Bratislava-Praha",
isbn="978-80-971422-0-9"
}