Detail publikace

DNA isolation by magnetic particles – first step in identification of microorganisms in food matrices

CHROBOKOVÁ, M. RITTICH, B. ŠPANOVÁ, A. HORÁK, D.

Originální název

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

Isolation of DNA is an important step in the molecular diagnostics of microorganisms. A high quality of isolated DNA is necessary for DNA amplification by the polymerase chain reaction (PCR). Total DNA was successfully isolated from cell lysates using magnetic P(HEMA-co-EDMA) and P(HEMA-co-GMA) microspheres covered by carboxyl groups in the presence of PEG 6000 and sodium chloride. DNA isolation by magnetic particles is comparable to the traditional extraction procedures such as phenol/chloroform one and non-magnetic silica particles but with the added advantages of reduced organic solvent consumption and processing time.

Klíčová slova

magnetic particles, DNA, microorganisms, foods; polymerase chain reaction

Autoři

CHROBOKOVÁ, M.; RITTICH, B.; ŠPANOVÁ, A.; HORÁK, D.

Rok RIV

2013

Vydáno

21. 3. 2013

Nakladatel

Československá spoločnosť mikrobiologická

Místo

Bratislava-Praha

ISBN

978-80-971422-0-9

Kniha

Čo nového v mikrobiologii

Strany od

Strany do

Strany počet

222

BibTex

@inproceedings{BUT99830,
  author="Maria {Chroboková} and Bohuslav {Rittich} and Alena {Španová} and Daniel {Horák}",
  title="DNA isolation by magnetic particles – first step in identification of microorganisms in food matrices",
  booktitle="Čo nového v mikrobiologii",
  year="2013",
  pages="94--98",
  publisher="Československá spoločnosť mikrobiologická",
  address="Bratislava-Praha",
  isbn="978-80-971422-0-9"
}