Publication detail
. Optimization of lipase production by Bacillus Optimization of lipase production by Bacillus subtilis
VOBĚRKOVÁ, S. HERMANOVÁ, S. OMELKOVÁ, J. SLAVÍČKOVÁ, R. RICHTERA, L.
Original Title
. Optimization of lipase production by Bacillus Optimization of lipase production by Bacillus subtilis
Type
abstract
Language
English
Original Abstract
The aim of this work was to study the effect of pH and temperature on the activity of lipase produced by the submerged culture of Bacillus subtilis CCM 1999. A series of cultivation media differing in the carbon sources and the nutrient content; brain heart infusion broth, nutrient broth and nutrient broth supplemented with 2 % glucose was used. The activity of lipase was measured spectrophotometrically using p-nitrophenyl laurate in ethanol as the substrate.
Keywords
Bacillus subtilis
Authors
VOBĚRKOVÁ, S.; HERMANOVÁ, S.; OMELKOVÁ, J.; SLAVÍČKOVÁ, R.; RICHTERA, L.
Released
17. 8. 2011
Publisher
Česká společnost chemická
Location
Praha
ISBN
0009-2770
Periodical
Chemické listy
Year of study
105 (S)
Number
Symposia
State
Czech Republic
Pages from
s1003
Pages to
s1004
Pages count
1