Publication detail
Evaluation of applicability of indirect photometric detection for chiral separations of selected carboxylic acids in dairy products
DVOŘÁK, M. VESPALCOVÁ, M.
Original Title
Evaluation of applicability of indirect photometric detection for chiral separations of selected carboxylic acids in dairy products
English Title
Evaluation of applicability of indirect photometric detection for chiral separations of selected carboxylic acids in dairy products
Type
conference paper
Language
Czech
Original Abstract
This work is check possibility application indirect photometric detection for enantiomers separation lactic acids and beta-hydroxybutyric acid with capillary electrophoresis. As chiral selector is used vankomycin. Verified his ability partial separation lactic acid on antipode with using picric acid as vizualition anion in background electrolyte. Used was coated capillary. Application indirect photometric detection succeed. Chiral separation beta-hydroxybutyric acid wasn't successful. They are selection suitable vizualization anions for indirect photometric detection. Are measured their electrophoretic and spectrophotometric characteristics, for determination compatibility use in capillary electrophoresis in relation with indirect photometric detection. From measured and searched characteristic was choice visualizations anions for next experimentation. According to ascertained and measured feature reflect be optimal eosine soluble in alcohol and bromophenol blue.
English abstract
This work is check possibility application indirect photometric detection for enantiomers separation lactic acids and beta-hydroxybutyric acid with capillary electrophoresis. As chiral selector is used vankomycin. Verified his ability partial separation lactic acid on antipode with using picric acid as vizualition anion in background electrolyte. Used was coated capillary. Application indirect photometric detection succeed. Chiral separation beta-hydroxybutyric acid wasn't successful. They are selection suitable vizualization anions for indirect photometric detection. Are measured their electrophoretic and spectrophotometric characteristics, for determination compatibility use in capillary electrophoresis in relation with indirect photometric detection. From measured and searched characteristic was choice visualizations anions for next experimentation. According to ascertained and measured feature reflect be optimal eosine soluble in alcohol and bromophenol blue.
Keywords
Capillary electrophoresis, chiral separation, lactic acid,
Authors
DVOŘÁK, M.; VESPALCOVÁ, M.
Released
3. 12. 2009
Publisher
Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno
Location
Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno
ISBN
978-80-214-3555-1
Book
STUDENTSKÁ ODBORNÁ KONFERENCE CHEMIE A SPOLEČNOST 2009 Sborník příspěvků
Edition
1
Edition number
1
Pages from
54
Pages to
55
Pages count
2
BibTex
@inproceedings{BUT65944,
author="Miloš {Dvořák} and Milena {Vespalcová}",
title="Evaluation of applicability of indirect photometric detection for chiral separations of selected carboxylic acids in dairy products",
booktitle="STUDENTSKÁ ODBORNÁ KONFERENCE CHEMIE A SPOLEČNOST 2009 Sborník příspěvků",
year="2009",
series="1",
number="1",
pages="54--55",
publisher="Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno",
address="Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno",
isbn="978-80-214-3555-1"
}