Evaluation of applicability of indirect photometric detection for chiral separations of selected carboxylic acids in dairy products

Evaluation of applicability of indirect photometric detection for chiral separations of selected carboxylic acids in dairy products

článek ve sborníku ve WoS nebo Scopus

čeština

This work is check possibility application indirect photometric detection for enantiomers separation lactic acids and beta-hydroxybutyric acid with capillary electrophoresis. As chiral selector is used vankomycin. Verified his ability partial separation lactic acid on antipode with using picric acid as vizualition anion in background electrolyte. Used was coated capillary. Application indirect photometric detection succeed. Chiral separation beta-hydroxybutyric acid wasn't successful. They are selection suitable vizualization anions for indirect photometric detection. Are measured their electrophoretic and spectrophotometric characteristics, for determination compatibility use in capillary electrophoresis in relation with indirect photometric detection. From measured and searched characteristic was choice visualizations anions for next experimentation. According to ascertained and measured feature reflect be optimal eosine soluble in alcohol and bromophenol blue.

This work is check possibility application indirect photometric detection for enantiomers separation lactic acids and beta-hydroxybutyric acid with capillary electrophoresis. As chiral selector is used vankomycin. Verified his ability partial separation lactic acid on antipode with using picric acid as vizualition anion in background electrolyte. Used was coated capillary. Application indirect photometric detection succeed. Chiral separation beta-hydroxybutyric acid wasn't successful. They are selection suitable vizualization anions for indirect photometric detection. Are measured their electrophoretic and spectrophotometric characteristics, for determination compatibility use in capillary electrophoresis in relation with indirect photometric detection. From measured and searched characteristic was choice visualizations anions for next experimentation. According to ascertained and measured feature reflect be optimal eosine soluble in alcohol and bromophenol blue.

Capillary electrophoresis, chiral separation, lactic acid,

DVOŘÁK, M.; VESPALCOVÁ, M.

3. 12. 2009

Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno

Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno

978-80-214-3555-1

STUDENTSKÁ ODBORNÁ KONFERENCE CHEMIE A SPOLEČNOST 2009 Sborník příspěvků

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