Publication detail

Non-selective and selective isolation of DNA from food matrices and other real samples by magnetic particles

ŠPANOVÁ, A., TVRDÍKOVÁ, J., HORÁK, D., RITTICH, B.

Original Title

Type

abstract

Language

English

Original Abstract

Recent research has been focused on the development of innovative magnetic micrometer-sized particles for specific applications in DNA analysis in food matrices and other real samples.Carboxyl group-containing magnetic nonporous hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-co-GMA)) microspheres were used for non-selective isolation of whole DNA from the sample. This procedure was used for PCR-ready DNA isolation and identification of Lactobacillus, Bifidobacterium and psychrotrophic bacteria in different dairy products or sediments. The magnetic microparticles P(HEMA-co-GMA) functionalised with streptavidin were used for immobilisation of biotinylated DNA probe and for selective isolation of target DNA from complex samples (e.g. food supplements) using DNA/DNA hybridisation. Isolation of target DNA was verified by PCR using genus-specific primers R16-1 and LblMA-rev (Dubernet et al., 2002) in food supplements.

Keywords

magnetic particles; streptavidin; DNA; PCR

Authors

ŠPANOVÁ, A., TVRDÍKOVÁ, J., HORÁK, D., RITTICH, B.

Released

4. 12. 2009

Publisher

Formatex, Španělsko

Location

Lisabon, Portugalsko

ISBN

978-80-210-4526-2

Pages from

327

Pages to

327

Pages count

BibTex

@misc{BUT61056,
  author="Alena {Španová} and Jana {Tvrdíková} and Bohuslav {Rittich}",
  title="Non-selective and selective isolation of DNA from food matrices and other real samples by magnetic particles",
  year="2009",
  pages="327--327",
  publisher="Formatex, Španělsko",
  address="Lisabon, Portugalsko",
  isbn="978-80-210-4526-2",
  note="abstract"
}