Detail publikace

Non-selective and selective isolation of DNA from food matrices and other real samples by magnetic particles

ŠPANOVÁ, A., TVRDÍKOVÁ, J., HORÁK, D., RITTICH, B.

Originální název

Typ

abstrakt

Jazyk

angličtina

Originální abstrakt

Recent research has been focused on the development of innovative magnetic micrometer-sized particles for specific applications in DNA analysis in food matrices and other real samples.Carboxyl group-containing magnetic nonporous hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-co-GMA)) microspheres were used for non-selective isolation of whole DNA from the sample. This procedure was used for PCR-ready DNA isolation and identification of Lactobacillus, Bifidobacterium and psychrotrophic bacteria in different dairy products or sediments. The magnetic microparticles P(HEMA-co-GMA) functionalised with streptavidin were used for immobilisation of biotinylated DNA probe and for selective isolation of target DNA from complex samples (e.g. food supplements) using DNA/DNA hybridisation. Isolation of target DNA was verified by PCR using genus-specific primers R16-1 and LblMA-rev (Dubernet et al., 2002) in food supplements.

Klíčová slova

magnetic particles; streptavidin; DNA; PCR

Autoři

ŠPANOVÁ, A., TVRDÍKOVÁ, J., HORÁK, D., RITTICH, B.

Vydáno

4. 12. 2009

Nakladatel

Formatex, Španělsko

Místo

Lisabon, Portugalsko

ISBN

978-80-210-4526-2

Strany od

327

Strany do

327

Strany počet

BibTex

@misc{BUT61056,
  author="Alena {Španová} and Jana {Tvrdíková} and Bohuslav {Rittich}",
  title="Non-selective and selective isolation of DNA from food matrices and other real samples by magnetic particles",
  year="2009",
  pages="327--327",
  publisher="Formatex, Španělsko",
  address="Lisabon, Portugalsko",
  isbn="978-80-210-4526-2",
  note="abstract"
}