Publication detail

Newly synthesized magnetic particles P (HEMA-co-GMA) for DNA isolation from probiotic strain Lactobacillus gasseri K7

TRACHTOVÁ, S. RITTICH, B. SPANOVA, A. HORAK, D. KUBISZ, P.

Original Title

Type

abstract

Language

English

Original Abstract

Probiotic lactic acid bacteria play an important role in the health maintenance of human gastrointestinal tract. Lactobacillus gasseri K7 is a probiotic strain which was isolated from feces of one week-old breast-fad baby [1]. Its probiotic characteristics were shown in more publications [1, 2]. The carboxyl group-containing magnetic nonporous poly(2-hydroxyethyl methacrylate-co-glycidyl dimethylmethacrylate) (P(HEMA-co-GMA) particles were used for the isolation of total DNA from pure culture Lactobacillus gasseri K7. Two types of magnetic particles with different concentration of carboxyl groups on their surface were tested. These particles reversibly bind DNA in the presence of high concentrations of poly(ethylene glycol) (PEG 6000) and sodium chloride [3].

Keywords

Magnetic particles P(HEMA-co-GMA), Lactobacillus gasseri K7, PCR

Authors

TRACHTOVÁ, S.; RITTICH, B.; SPANOVA, A.; HORAK, D.; KUBISZ, P.

Released

23. 6. 2010

Publisher

Pamida International Ltd.

Location

Kysucke Nove Mesto, Slovak Republic

ISBN

978-80-970168-4-5

Book

International Scientific Conference Probiotics and Prebiotics Conference Proceedings

Pages from

158

Pages to

158

Pages count

BibTex

@misc{BUT60995,
  author="Štěpánka {Trachtová} and Bohuslav {Rittich} and Alena {Španová} and Daniel {Horák} and Petr {Kubisz}",
  title="Newly synthesized magnetic particles P (HEMA-co-GMA) for DNA isolation from probiotic strain Lactobacillus gasseri K7",
  booktitle="International Scientific Conference Probiotics and Prebiotics Conference Proceedings",
  year="2010",
  pages="158--158",
  publisher="Pamida International Ltd.",
  address="Kysucke Nove Mesto, Slovak Republic",
  isbn="978-80-970168-4-5",
  note="abstract"
}