Detail publikace

Newly synthesized magnetic particles P (HEMA-co-GMA) for DNA isolation from probiotic strain Lactobacillus gasseri K7

TRACHTOVÁ, S. RITTICH, B. SPANOVA, A. HORAK, D. KUBISZ, P.

Originální název

Typ

abstrakt

Jazyk

angličtina

Originální abstrakt

Probiotic lactic acid bacteria play an important role in the health maintenance of human gastrointestinal tract. Lactobacillus gasseri K7 is a probiotic strain which was isolated from feces of one week-old breast-fad baby [1]. Its probiotic characteristics were shown in more publications [1, 2]. The carboxyl group-containing magnetic nonporous poly(2-hydroxyethyl methacrylate-co-glycidyl dimethylmethacrylate) (P(HEMA-co-GMA) particles were used for the isolation of total DNA from pure culture Lactobacillus gasseri K7. Two types of magnetic particles with different concentration of carboxyl groups on their surface were tested. These particles reversibly bind DNA in the presence of high concentrations of poly(ethylene glycol) (PEG 6000) and sodium chloride [3].

Klíčová slova

Magnetic particles P(HEMA-co-GMA), Lactobacillus gasseri K7, PCR

Autoři

TRACHTOVÁ, S.; RITTICH, B.; SPANOVA, A.; HORAK, D.; KUBISZ, P.

Vydáno

23. 6. 2010

Nakladatel

Pamida International Ltd.

Místo

Kysucke Nove Mesto, Slovak Republic

ISBN

978-80-970168-4-5

Kniha

International Scientific Conference Probiotics and Prebiotics Conference Proceedings

Strany od

158

Strany do

158

Strany počet

BibTex

@misc{BUT60995,
  author="Štěpánka {Trachtová} and Bohuslav {Rittich} and Alena {Španová} and Daniel {Horák} and Petr {Kubisz}",
  title="Newly synthesized magnetic particles P (HEMA-co-GMA) for DNA isolation from probiotic strain Lactobacillus gasseri K7",
  booktitle="International Scientific Conference Probiotics and Prebiotics Conference Proceedings",
  year="2010",
  pages="158--158",
  publisher="Pamida International Ltd.",
  address="Kysucke Nove Mesto, Slovak Republic",
  isbn="978-80-970168-4-5",
  note="abstract"
}