Publication detail

Isolation of poly(3-hydroxybutyrate) from bacterial biomass using soap made of waste cooking oil

POSPÍŠILOVÁ, A. NOVÁČKOVÁ, I. PŘIKRYL, R.

Original Title

Type

journal article in Web of Science

Language

English

Original Abstract

The aim of this work was to develop a soap-based method for the isolation of poly(3-hydroxybutyrate) from bacterial biomass. The method consisted of adding soap derived from waste cooking oil to a concentrated (25%) biomass suspension, heating and centrifugal separation. Purity above 95% could be achieved with soap:cell dry mass ratios at least 0.125 g/g, making the method comparable to other surfactant-based protocols. Molecular weights M, of products from all experiments were between 350 and 450 kDa, being high enough for future material applications. Addition of hydrochloric acid to the wastewater led to the precipitation of soap and part of non-P3HB cell mass. The resulting precipitate was utilized as a carbon source in biomass production and increased substrate-to-P3HB conversion.

Keywords

PHA; P3HB; Recovery; Downstream process; Isolation

Authors

POSPÍŠILOVÁ, A.; NOVÁČKOVÁ, I.; PŘIKRYL, R.

Released

1. 4. 2021

Publisher

Elsevier

Location

OXFORD

ISBN

0960-8524

Periodical

BIORESOURCE TECHNOLOGY

Year of study

326

Number

State

Kingdom of the Netherlands

Pages from

Pages to

Pages count

URL

https://www.sciencedirect.com/science/article/pii/S0960852421000213

Full text in the Digital Library

http://hdl.handle.net/11012/203003

BibTex

@article{BUT171646,
  author="Aneta {Pospíšilová} and Ivana {Nováčková} and Radek {Přikryl}",
  title="Isolation of poly(3-hydroxybutyrate) from bacterial biomass using soap made of waste cooking oil",
  journal="BIORESOURCE TECHNOLOGY",
  year="2021",
  volume="326",
  number="1",
  pages="1--5",
  doi="10.1016/j.biortech.2021.124683",
  issn="0960-8524",
  url="https://www.sciencedirect.com/science/article/pii/S0960852421000213"
}