Detail publikace

Isolation of poly(3-hydroxybutyrate) from bacterial biomass using soap made of waste cooking oil

POSPÍŠILOVÁ, A. NOVÁČKOVÁ, I. PŘIKRYL, R.

Originální název

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The aim of this work was to develop a soap-based method for the isolation of poly(3-hydroxybutyrate) from bacterial biomass. The method consisted of adding soap derived from waste cooking oil to a concentrated (25%) biomass suspension, heating and centrifugal separation. Purity above 95% could be achieved with soap:cell dry mass ratios at least 0.125 g/g, making the method comparable to other surfactant-based protocols. Molecular weights M, of products from all experiments were between 350 and 450 kDa, being high enough for future material applications. Addition of hydrochloric acid to the wastewater led to the precipitation of soap and part of non-P3HB cell mass. The resulting precipitate was utilized as a carbon source in biomass production and increased substrate-to-P3HB conversion.

Klíčová slova

PHA; P3HB; Recovery; Downstream process; Isolation

Autoři

POSPÍŠILOVÁ, A.; NOVÁČKOVÁ, I.; PŘIKRYL, R.

Vydáno

1. 4. 2021

Nakladatel

Elsevier

Místo

OXFORD

ISSN

0960-8524

Periodikum

BIORESOURCE TECHNOLOGY

Ročník

326

Číslo

Stát

Nizozemsko

Strany od

Strany do

Strany počet

URL

https://www.sciencedirect.com/science/article/pii/S0960852421000213

Plný text v Digitální knihovně

http://hdl.handle.net/11012/203003

BibTex

@article{BUT171646,
  author="Aneta {Pospíšilová} and Ivana {Nováčková} and Radek {Přikryl}",
  title="Isolation of poly(3-hydroxybutyrate) from bacterial biomass using soap made of waste cooking oil",
  journal="BIORESOURCE TECHNOLOGY",
  year="2021",
  volume="326",
  number="1",
  pages="1--5",
  doi="10.1016/j.biortech.2021.124683",
  issn="0960-8524",
  url="https://www.sciencedirect.com/science/article/pii/S0960852421000213"
}