Publication detail
Approaches of evolutionary engineering for adaptation of Cupriavidus necator H16 to biotechnologically relevant stressors
NOVÁČKOVÁ, I. CHATRNÁ, V. SLANINOVÁ, E. SEDLÁČEK, P. SAMEK, O. OBRUČA, S.
Original Title
Approaches of evolutionary engineering for adaptation of Cupriavidus necator H16 to biotechnologically relevant stressors
Type
abstract
Language
English
Original Abstract
Model PHA producer Cupriavidus necator H16 (CCM 3726) was exposed to presence of NaCl causing osmotic stress which represented environmental stress factor, to copper ions representing anthropogenic pollutant and levulinic acid, microbial inhibitor commonly presented in hydrolysates of lignocellulosic biomass. Multiple serial transfers of cell cultures in Erlenmeyer flasks after 48 hours were provided within adaptation experiments for more than 80 passages. Passages have been basically characterized (growth potential, analysis of PHAs) and selected preserved ones were also compared with wild-type strain considering potential of PHAs accumulation (GC-FID), testing of robustness, “fingerprint” of cells using Raman spectroscopy and others.
Keywords
evolutionary engineering, Cupriavidus necator H16, osmotic stress, copper ions
Authors
NOVÁČKOVÁ, I.; CHATRNÁ, V.; SLANINOVÁ, E.; SEDLÁČEK, P.; SAMEK, O.; OBRUČA, S.
Released
15. 6. 2020
Publisher
Masarykova univerzita
Location
Brno
ISBN
978-80-210-9611-0
Book
XXIX. konference mladých mikrobiologů TOMÁŠKOVY DNY 2020
Edition
1
Edition number
1
Pages from
45
Pages to
45
Pages count
1
BibTex
@misc{BUT164450,
author="Ivana {Nováčková} and Vendula {Hrabalová} and Eva {Slaninová} and Petr {Sedláček} and Ota {Samek} and Stanislav {Obruča}",
title="Approaches of evolutionary engineering for adaptation of Cupriavidus necator H16 to biotechnologically relevant stressors",
booktitle="XXIX. konference mladých mikrobiologů TOMÁŠKOVY DNY 2020",
year="2020",
series="1",
edition="1",
pages="45--45",
publisher="Masarykova univerzita",
address="Brno",
isbn="978-80-210-9611-0",
note="abstract"
}