Detail publikace

Approaches of evolutionary engineering for adaptation of Cupriavidus necator H16 to biotechnologically relevant stressors

NOVÁČKOVÁ, I. CHATRNÁ, V. SLANINOVÁ, E. SEDLÁČEK, P. SAMEK, O. OBRUČA, S.

Originální název

Approaches of evolutionary engineering for adaptation of Cupriavidus necator H16 to biotechnologically relevant stressors

Typ

abstrakt

Jazyk

angličtina

Originální abstrakt

Model PHA producer Cupriavidus necator H16 (CCM 3726) was exposed to presence of NaCl causing osmotic stress which represented environmental stress factor, to copper ions representing anthropogenic pollutant and levulinic acid, microbial inhibitor commonly presented in hydrolysates of lignocellulosic biomass. Multiple serial transfers of cell cultures in Erlenmeyer flasks after 48 hours were provided within adaptation experiments for more than 80 passages. Passages have been basically characterized (growth potential, analysis of PHAs) and selected preserved ones were also compared with wild-type strain considering potential of PHAs accumulation (GC-FID), testing of robustness, “fingerprint” of cells using Raman spectroscopy and others.

Klíčová slova

evolutionary engineering, Cupriavidus necator H16, osmotic stress, copper ions

Autoři

NOVÁČKOVÁ, I.; CHATRNÁ, V.; SLANINOVÁ, E.; SEDLÁČEK, P.; SAMEK, O.; OBRUČA, S.

Vydáno

15. 6. 2020

Nakladatel

Masarykova univerzita

Místo

Brno

ISBN

978-80-210-9611-0

Kniha

XXIX. konference mladých mikrobiologů TOMÁŠKOVY DNY 2020

Edice

1

Číslo edice

1

Strany od

45

Strany do

45

Strany počet

1

BibTex

@misc{BUT164450,
  author="Ivana {Nováčková} and Vendula {Hrabalová} and Eva {Slaninová} and Petr {Sedláček} and Ota {Samek} and Stanislav {Obruča}",
  title="Approaches of evolutionary engineering for adaptation of Cupriavidus necator H16 to biotechnologically relevant stressors",
  booktitle="XXIX. konference mladých mikrobiologů TOMÁŠKOVY DNY 2020",
  year="2020",
  series="1",
  edition="1",
  pages="45--45",
  publisher="Masarykova univerzita",
  address="Brno",
  isbn="978-80-210-9611-0",
  note="abstract"
}