Publication detail

Toxicity evaluation of monodisperse PEGylated magnetic nanoparticles for nanomedicine

Patsula, V. Tulinska, J. Trachtova, S. Kuricova, M. Liskova, A. Spanova, A. Ciampor, F. Vavra, I. Rittich, B. Ursinyova, M. Dusinska, M. Ilayska, S. Horvathova, M. Masanova, V. Uhnakova, I. Horak, D.

Original Title

Type

journal article in Web of Science

Language

English

Original Abstract

Innovative nanotechnology aims to develop particles that are small, monodisperse, smart, and do not cause unintentional side effects. Uniform magnetic Fe3O4 nanoparticles (12nm in size) were prepared by thermal decomposition of iron(III) oleate. To make them colloidally stable and dispersible in water and cell culture medium, they were modified with phosphonic acid- (PA) and hydroxamic acid (HA)-terminated poly(ethylene glycol) yielding PA-PEG@Fe3O4 and HA-PEG@Fe3O4 nanoparticles; conventional gamma-Fe2O3 particles were prepared as a control. Advanced techniques were used to evaluate the properties and safety of the particles. Completeness of the nanoparticle coating was tested by real-time polymerase chain reaction. Interaction of the particles with primary human peripheral blood cells, cellular uptake, cytotoxicity, and immunotoxicity were also investigated. Amount of internalized iron in peripheral blood mononuclear cells was 72, 38, and 25pg Fe/cell for HA-PEG@Fe3O4, gamma-Fe2O3, and PA-PEG@Fe3O4, respectively. Nanoparticles were localized within the cytoplasm and in the extracellular space. No cytotoxic effect of both PEGylated nanoparticles was observed (0.12-75 mu g/cm(2)) after 24 and 72-h incubation. Moreover, no suppressive effect was found on the proliferative activity of T-lymphocytes and T-dependent B-cell response, phagocytic activity of monocytes and granulocytes, and respiratory burst of phagocytes. Similarly, no cytotoxic effect of gamma-Fe2O3 particles was observed. However, they suppressed the proliferative activity of T-lymphocytes (75 mu g/cm(2), 72h) and also decreased the phagocytic activity of monocytes (15 mu g/cm(2), 24h; 3-75 mu g/cm(2), 72h). We thus show that newly developed particles have great potential especially in cancer diagnostics and therapy

Keywords

Iron oxide nanoparticles; PCR inhibition; cell interaction and uptake; cytotoxicity and immunotoxicity; human peripheral blood leucocytes

Authors

Patsula, V. ; Tulinska, J.; Trachtova, S.; Kuricova, M.; Liskova, A. ; Spanova, A.; Ciampor, F.; Vavra, I.; Rittich, B.; Ursinyova, M.; Dusinska, M. ; Ilayska, S.; Horvathova, M.; Masanova, V.; Uhnakova, I.; Horak, D.

Released

21. 4. 2019

Publisher

TAYLOR & FRANCIS LTD

ISBN

1743-5390

Periodical

NANOTOXICOLOGY

Year of study

Number

State

United Kingdom of Great Britain and Northern Ireland

Pages from

510

Pages to

526

Pages count

URL

https://www.tandfonline.com/doi/abs/10.1080/17435390.2018.1555624?scroll=top&needAccess=true&journalCode=inan20

BibTex

@article{BUT163527,
  author="Patsula, V. and Tulinska, J. and Trachtova, S. and Kuricova, M. and Liskova, A. and Spanova, A. and Ciampor, F. and Vavra, I. and Rittich, B. and Ursinyova, M. and Dusinska, M. and Ilayska, S. and Horvathova, M. and Masanova, V. and Uhnakova, I. and Horak, D.",
  title="Toxicity evaluation of monodisperse PEGylated magnetic nanoparticles for nanomedicine",
  journal="NANOTOXICOLOGY",
  year="2019",
  volume="13",
  number="4",
  pages="510--526",
  doi="10.1080/17435390.2018.1555624",
  issn="1743-5390",
  url="https://www.tandfonline.com/doi/abs/10.1080/17435390.2018.1555624?scroll=top&needAccess=true&journalCode=inan20"
}