Detail publikace

Toxicity evaluation of monodisperse PEGylated magnetic nanoparticles for nanomedicine

Patsula, V. Tulinska, J. Trachtova, S. Kuricova, M. Liskova, A. Spanova, A. Ciampor, F. Vavra, I. Rittich, B. Ursinyova, M. Dusinska, M. Ilayska, S. Horvathova, M. Masanova, V. Uhnakova, I. Horak, D.

Originální název

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Innovative nanotechnology aims to develop particles that are small, monodisperse, smart, and do not cause unintentional side effects. Uniform magnetic Fe3O4 nanoparticles (12nm in size) were prepared by thermal decomposition of iron(III) oleate. To make them colloidally stable and dispersible in water and cell culture medium, they were modified with phosphonic acid- (PA) and hydroxamic acid (HA)-terminated poly(ethylene glycol) yielding PA-PEG@Fe3O4 and HA-PEG@Fe3O4 nanoparticles; conventional gamma-Fe2O3 particles were prepared as a control. Advanced techniques were used to evaluate the properties and safety of the particles. Completeness of the nanoparticle coating was tested by real-time polymerase chain reaction. Interaction of the particles with primary human peripheral blood cells, cellular uptake, cytotoxicity, and immunotoxicity were also investigated. Amount of internalized iron in peripheral blood mononuclear cells was 72, 38, and 25pg Fe/cell for HA-PEG@Fe3O4, gamma-Fe2O3, and PA-PEG@Fe3O4, respectively. Nanoparticles were localized within the cytoplasm and in the extracellular space. No cytotoxic effect of both PEGylated nanoparticles was observed (0.12-75 mu g/cm(2)) after 24 and 72-h incubation. Moreover, no suppressive effect was found on the proliferative activity of T-lymphocytes and T-dependent B-cell response, phagocytic activity of monocytes and granulocytes, and respiratory burst of phagocytes. Similarly, no cytotoxic effect of gamma-Fe2O3 particles was observed. However, they suppressed the proliferative activity of T-lymphocytes (75 mu g/cm(2), 72h) and also decreased the phagocytic activity of monocytes (15 mu g/cm(2), 24h; 3-75 mu g/cm(2), 72h). We thus show that newly developed particles have great potential especially in cancer diagnostics and therapy

Klíčová slova

Iron oxide nanoparticles; PCR inhibition; cell interaction and uptake; cytotoxicity and immunotoxicity; human peripheral blood leucocytes

Autoři

Patsula, V. ; Tulinska, J.; Trachtova, S.; Kuricova, M.; Liskova, A. ; Spanova, A.; Ciampor, F.; Vavra, I.; Rittich, B.; Ursinyova, M.; Dusinska, M. ; Ilayska, S.; Horvathova, M.; Masanova, V.; Uhnakova, I.; Horak, D.

Vydáno

21. 4. 2019

Nakladatel

TAYLOR & FRANCIS LTD

ISSN

1743-5390

Periodikum

NANOTOXICOLOGY

Ročník

Číslo

Stát

Spojené království Velké Británie a Severního Irska

Strany od

510

Strany do

526

Strany počet

URL

https://www.tandfonline.com/doi/abs/10.1080/17435390.2018.1555624?scroll=top&needAccess=true&journalCode=inan20

BibTex

@article{BUT163527,
  author="Patsula, V. and Tulinska, J. and Trachtova, S. and Kuricova, M. and Liskova, A. and Spanova, A. and Ciampor, F. and Vavra, I. and Rittich, B. and Ursinyova, M. and Dusinska, M. and Ilayska, S. and Horvathova, M. and Masanova, V. and Uhnakova, I. and Horak, D.",
  title="Toxicity evaluation of monodisperse PEGylated magnetic nanoparticles for nanomedicine",
  journal="NANOTOXICOLOGY",
  year="2019",
  volume="13",
  number="4",
  pages="510--526",
  doi="10.1080/17435390.2018.1555624",
  issn="1743-5390",
  url="https://www.tandfonline.com/doi/abs/10.1080/17435390.2018.1555624?scroll=top&needAccess=true&journalCode=inan20"
}