Publication detail

Protective effect of PHA granules in bacterial cells against UV-irradiation investigated by various spectral techniques

SLANINOVÁ, E. SEDLÁČEK, P. MRAVEC, F. HESKO, O. MÜLLEROVÁ, L. OBRUČA, S.

Original Title

Type

abstract

Language

English

Original Abstract

Polyhydroxyalkanoates (PHA) are polyesters accumulated in form of intracellular granules by numerous bacteria primarily as storage compounds, nevertheless, we investigated that PHA granules are not used only as carbon and energy storage but bacteria can use this compound also as UV-protectant. This statement is based on our observation that PHA accumulating cells of Cupriavidus necator H16 survived exposure to UV irradiation challenge much better than its PHA non-accumulating mutant. Mechanisms of protective effect of PHA granules were determined by different spectroscopic approaches such as turbidity measurement, absorption spectrophotometry with integration sphere, nephelometry and continuous fluorescence microphotolysis. Generally, protective mechanisms of PHA granules is predominantly based on very effective scattering of UV radiation with wavelength of about 250 – 290 nm.

Keywords

polyhydroxyalkanoates, granules, UV irradiation, protective effect, spectrophotometry

Authors

SLANINOVÁ, E.; SEDLÁČEK, P.; MRAVEC, F.; HESKO, O.; MÜLLEROVÁ, L.; OBRUČA, S.

Released

25. 9. 2019

Publisher

C.A.R.M.E.V. e.V.

Location

Straubing

Pages from

Pages to

Pages count

114

BibTex

@misc{BUT159300,
  author="Eva {Slaninová} and Petr {Sedláček} and Filip {Mravec} and Ondrej {Hesko} and Lucie {Müllerová} and Stanislav {Obruča}",
  title="Protective effect of PHA granules in bacterial cells against UV-irradiation investigated by various spectral techniques",
  year="2019",
  pages="88--88",
  publisher="C.A.R.M.E.V. e.V.",
  address="Straubing",
  note="abstract"
}