Detail publikace

Protective effect of PHA granules in bacterial cells against UV-irradiation investigated by various spectral techniques

SLANINOVÁ, E. SEDLÁČEK, P. MRAVEC, F. HESKO, O. MÜLLEROVÁ, L. OBRUČA, S.

Originální název

Typ

abstrakt

Jazyk

angličtina

Originální abstrakt

Polyhydroxyalkanoates (PHA) are polyesters accumulated in form of intracellular granules by numerous bacteria primarily as storage compounds, nevertheless, we investigated that PHA granules are not used only as carbon and energy storage but bacteria can use this compound also as UV-protectant. This statement is based on our observation that PHA accumulating cells of Cupriavidus necator H16 survived exposure to UV irradiation challenge much better than its PHA non-accumulating mutant. Mechanisms of protective effect of PHA granules were determined by different spectroscopic approaches such as turbidity measurement, absorption spectrophotometry with integration sphere, nephelometry and continuous fluorescence microphotolysis. Generally, protective mechanisms of PHA granules is predominantly based on very effective scattering of UV radiation with wavelength of about 250 – 290 nm.

Klíčová slova

polyhydroxyalkanoates, granules, UV irradiation, protective effect, spectrophotometry

Autoři

SLANINOVÁ, E.; SEDLÁČEK, P.; MRAVEC, F.; HESKO, O.; MÜLLEROVÁ, L.; OBRUČA, S.

Vydáno

25. 9. 2019

Nakladatel

C.A.R.M.E.V. e.V.

Místo

Straubing

Strany od

Strany do

Strany počet

114

BibTex

@misc{BUT159300,
  author="Eva {Slaninová} and Petr {Sedláček} and Filip {Mravec} and Ondrej {Hesko} and Lucie {Müllerová} and Stanislav {Obruča}",
  title="Protective effect of PHA granules in bacterial cells against UV-irradiation investigated by various spectral techniques",
  year="2019",
  pages="88--88",
  publisher="C.A.R.M.E.V. e.V.",
  address="Straubing",
  note="abstract"
}