Publication detail
A simple technique for assessing the cuticular diffusion of humic acid biostimulants
SMILKOVÁ, M. SMILEK, J. KALINA, M. KLUČÁKOVÁ, M. PEKAŘ, M. SEDLÁČEK, P.
Original Title
A simple technique for assessing the cuticular diffusion of humic acid biostimulants
Type
journal article in Web of Science
Language
English
Original Abstract
Background: Experimental determination of the extent and rate of transport of liquid humates supplied to plants is critical in testing of physiological effects of such biostimulants which are often supplied as foliar sprays. Therefore, an original experimental method for the qualitative investigation and quantitative description of the penetration on humates through plant cuticles is proposed, tested, and evaluated. Results: The proposed method involves the isolation of model plant leaf cuticles and the subsequent in vitro evaluation of cuticular humate transport. The employed novel methodology is based on a simple diffusion couple arrangement involving continuous spectrophotometric determination of the amounts of penetrated humate in a hydrogel diffusion medium. Prunus laurocerasus leaf cuticles were isolated by chemical and enzymatic treatment and the rate of cuticular penetration of a commercial humate (lignohumate) was estimated over time in quantitative and qualitative terms. Different rates of lignohumate transport were determined for abaxial and adaxial leaf cuticles also in relation to the different cuticular extraction methods tested. Conclusions: The proposed methodology represents a simple and cheap experimental tool for the study on the trans-cuticular penetration of humic-based biostimulants.
Keywords
Diffusion, Hydrogel, Prunus laurocerasus, Liquid fertilization, Humic substances, Plant cuticle
Authors
SMILKOVÁ, M.; SMILEK, J.; KALINA, M.; KLUČÁKOVÁ, M.; PEKAŘ, M.; SEDLÁČEK, P.
Released
31. 7. 2019
Publisher
BMC
ISBN
1746-4811
Periodical
PLANT METHODS
Year of study
15
Number
1
State
United Kingdom of Great Britain and Northern Ireland
Pages from
1
Pages to
11
Pages count
11
URL
Full text in the Digital Library
BibTex
@article{BUT157782,
author="Marcela {Smilková} and Jiří {Smilek} and Michal {Kalina} and Martina {Klučáková} and Miloslav {Pekař} and Petr {Sedláček}",
title="A simple technique for assessing the cuticular diffusion of humic acid biostimulants",
journal="PLANT METHODS",
year="2019",
volume="15",
number="1",
pages="1--11",
doi="10.1186/s13007-019-0469-x",
issn="1746-4811",
url="https://plantmethods.biomedcentral.com/articles/10.1186/s13007-019-0469-x#Abs1"
}