Detail publikace

A simple technique for assessing the cuticular diffusion of humic acid biostimulants

SMILKOVÁ, M. SMILEK, J. KALINA, M. KLUČÁKOVÁ, M. PEKAŘ, M. SEDLÁČEK, P.

Originální název

A simple technique for assessing the cuticular diffusion of humic acid biostimulants

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Background: Experimental determination of the extent and rate of transport of liquid humates supplied to plants is critical in testing of physiological effects of such biostimulants which are often supplied as foliar sprays. Therefore, an original experimental method for the qualitative investigation and quantitative description of the penetration on humates through plant cuticles is proposed, tested, and evaluated. Results: The proposed method involves the isolation of model plant leaf cuticles and the subsequent in vitro evaluation of cuticular humate transport. The employed novel methodology is based on a simple diffusion couple arrangement involving continuous spectrophotometric determination of the amounts of penetrated humate in a hydrogel diffusion medium. Prunus laurocerasus leaf cuticles were isolated by chemical and enzymatic treatment and the rate of cuticular penetration of a commercial humate (lignohumate) was estimated over time in quantitative and qualitative terms. Different rates of lignohumate transport were determined for abaxial and adaxial leaf cuticles also in relation to the different cuticular extraction methods tested. Conclusions: The proposed methodology represents a simple and cheap experimental tool for the study on the trans-cuticular penetration of humic-based biostimulants.

Klíčová slova

Diffusion, Hydrogel, Prunus laurocerasus, Liquid fertilization, Humic substances, Plant cuticle

Autoři

SMILKOVÁ, M.; SMILEK, J.; KALINA, M.; KLUČÁKOVÁ, M.; PEKAŘ, M.; SEDLÁČEK, P.

Vydáno

31. 7. 2019

Nakladatel

BMC

ISSN

1746-4811

Periodikum

PLANT METHODS

Ročník

15

Číslo

1

Stát

Spojené království Velké Británie a Severního Irska

Strany od

1

Strany do

11

Strany počet

11

URL

https://plantmethods.biomedcentral.com/articles/10.1186/s13007-019-0469-x#Abs1

Plný text v Digitální knihovně

http://hdl.handle.net/11012/180811

BibTex

@article{BUT157782,
  author="Marcela {Smilková} and Jiří {Smilek} and Michal {Kalina} and Martina {Klučáková} and Miloslav {Pekař} and Petr {Sedláček}",
  title="A simple technique for assessing the cuticular diffusion of humic acid biostimulants",
  journal="PLANT METHODS",
  year="2019",
  volume="15",
  number="1",
  pages="1--11",
  doi="10.1186/s13007-019-0469-x",
  issn="1746-4811",
  url="https://plantmethods.biomedcentral.com/articles/10.1186/s13007-019-0469-x#Abs1"
}