Publication detail

Efficient non-enzymatic cleavage of Staphylococcus aureus plasmid DNAs mediated by neodymium ions

ZOVČÁKOVÁ, M. ŠPANOVÁ, A. PANTŮČEK, R. DOŠKAŘ, J. RITTICH, B.

Original Title

Type

journal article in Web of Science

Language

English

Original Abstract

Plasmids recovered from 12 clinical and food isolates of S. aureus were treated with 10 mM free lanthanide Nd3+ ions (non-enzymatic cleavage agent) in HEPES buffer (pH 7.5) at 70 °C. Topological forms of plasmids (ccc, oc, lin) produced by cleavage at different times were separated using pulsed-field agarose gel electrophoresis. The method is proposed to detect and differentiate several plasmids in the same bacterial strain according to their size.

Keywords

lanthanide Nd3+ ions; plasmid DNA; plasmid size; pulsed-field agarose gel electrophoresis; Staphylococcus aureus

Authors

ZOVČÁKOVÁ, M.; ŠPANOVÁ, A.; PANTŮČEK, R.; DOŠKAŘ, J.; RITTICH, B.

Released

26. 5. 2016

Publisher

Elsevier

Location

USA

ISBN

0003-2697

Periodical

ANALYTICAL BIOCHEMISTRY

Year of study

507

Number

State

United States of America

Pages from

Pages to

Pages count

BibTex

@article{BUT124880,
  author="Monika {Zovčáková} and Alena {Španová} and Roman {Pantůček} and Jiří {Doškař} and Bohuslav {Rittich}",
  title="Efficient non-enzymatic cleavage of Staphylococcus aureus plasmid DNAs mediated by neodymium ions",
  journal="ANALYTICAL BIOCHEMISTRY",
  year="2016",
  volume="507",
  number="1",
  pages="66--70",
  doi="10.1016/j.ab.2016.05.013",
  issn="0003-2697"
}