Detail publikace

Efficient non-enzymatic cleavage of Staphylococcus aureus plasmid DNAs mediated by neodymium ions

ZOVČÁKOVÁ, M. ŠPANOVÁ, A. PANTŮČEK, R. DOŠKAŘ, J. RITTICH, B.

Originální název

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Plasmids recovered from 12 clinical and food isolates of S. aureus were treated with 10 mM free lanthanide Nd3+ ions (non-enzymatic cleavage agent) in HEPES buffer (pH 7.5) at 70 °C. Topological forms of plasmids (ccc, oc, lin) produced by cleavage at different times were separated using pulsed-field agarose gel electrophoresis. The method is proposed to detect and differentiate several plasmids in the same bacterial strain according to their size.

Klíčová slova

lanthanide Nd3+ ions; plasmid DNA; plasmid size; pulsed-field agarose gel electrophoresis; Staphylococcus aureus

Autoři

ZOVČÁKOVÁ, M.; ŠPANOVÁ, A.; PANTŮČEK, R.; DOŠKAŘ, J.; RITTICH, B.

Vydáno

26. 5. 2016

Nakladatel

Elsevier

Místo

USA

ISSN

0003-2697

Periodikum

ANALYTICAL BIOCHEMISTRY

Ročník

507

Číslo

Stát

Spojené státy americké

Strany od

Strany do

Strany počet

BibTex

@article{BUT124880,
  author="Monika {Zovčáková} and Alena {Španová} and Roman {Pantůček} and Jiří {Doškař} and Bohuslav {Rittich}",
  title="Efficient non-enzymatic cleavage of Staphylococcus aureus plasmid DNAs mediated by neodymium ions",
  journal="ANALYTICAL BIOCHEMISTRY",
  year="2016",
  volume="507",
  number="1",
  pages="66--70",
  doi="10.1016/j.ab.2016.05.013",
  issn="0003-2697"
}