Publication detail
Optimisation of DGGE nested PCR for red yeasts characterisation
KOSTOVOVÁ, I. HÁRONIKOVÁ, A. PETRIK, S. MÁROVÁ, I.
Original Title
Optimisation of DGGE nested PCR for red yeasts characterisation
Type
abstract
Language
English
Original Abstract
Carotenogenic yeasts are a diverse group of unrelated organisms (mostly Basidiomycota) that can be found in soil, fresh and marine water, on plants, commonly associated with animals and man-made habitant. In this work the PCR - denaturing gradient gel electrophoresis (DGGE) method was optimized for characterisation of red yeast strains. For this usage, inter-specific variables of strongly conserved sequences of genomic DNA, especially ITS1 and 5.8-ITS2 rDNA regions were amplified. At all, nine of carotenogenic yeast strains of the genus Rhodotorula, Sporobolomyces, Cystofilobasidium and Phaffia were analyzed.
Keywords
DGGE; PCR; red yeast
Authors
KOSTOVOVÁ, I.; HÁRONIKOVÁ, A.; PETRIK, S.; MÁROVÁ, I.
Released
20. 5. 2014
Pages from
55
Pages to
55
Pages count
86
BibTex
@misc{BUT111756,
author="Iveta {Kostovová} and Andrea {Němcová} and Siniša {Petrik} and Ivana {Márová}",
title="Optimisation of DGGE nested PCR for red yeasts characterisation",
booktitle="41st annual conference on yeast",
year="2014",
pages="55--55",
note="abstract"
}