Detail publikace

Optimisation of DGGE nested PCR for red yeasts characterisation

KOSTOVOVÁ, I. HÁRONIKOVÁ, A. PETRIK, S. MÁROVÁ, I.

Originální název

Typ

abstrakt

Jazyk

angličtina

Originální abstrakt

Carotenogenic yeasts are a diverse group of unrelated organisms (mostly Basidiomycota) that can be found in soil, fresh and marine water, on plants, commonly associated with animals and man-made habitant. In this work the PCR - denaturing gradient gel electrophoresis (DGGE) method was optimized for characterisation of red yeast strains. For this usage, inter-specific variables of strongly conserved sequences of genomic DNA, especially ITS1 and 5.8-ITS2 rDNA regions were amplified. At all, nine of carotenogenic yeast strains of the genus Rhodotorula, Sporobolomyces, Cystofilobasidium and Phaffia were analyzed.

Klíčová slova

DGGE; PCR; red yeast

Autoři

KOSTOVOVÁ, I.; HÁRONIKOVÁ, A.; PETRIK, S.; MÁROVÁ, I.

Vydáno

20. 5. 2014

Strany od

Strany do

Strany počet

BibTex

@misc{BUT111756,
  author="Iveta {Kostovová} and Andrea {Němcová} and Siniša {Petrik} and Ivana {Márová}",
  title="Optimisation of DGGE nested PCR for red yeasts characterisation",
  booktitle="41st annual conference on yeast",
  year="2014",
  pages="55--55",
  note="abstract"
}