Publication detail

Application of protease-hydrolyzed whey as a complex nitrogen source to increase poly(3-hydroxybutyrate) production from oils by Cupriavidus necator

OBRUČA, S. BENEŠOVÁ, P. OBORNÁ, J. MÁROVÁ, I.

Original Title

Type

journal article in Web of Science

Language

English

Original Abstract

Whole whey hydrolyzed by Alcalase (WWH) was tested as a complex nitrogen source for the production of poly(3-hydroxybutyrate) (PHB) from waste frying oils by Cupriavidus necator H16. The addition of WWH (10 % (vol/vol) of cultivation media) supported the growth of the bacterial culture and PHB accumulation; hence the PHB yields achieved in Erlenmeyer flasks were more than 3.5-fold higher than in control cultivation. The positive influence of WWH on PHB production was confirmed in experiments performed in laboratory fermentor. C. necator cultivated in the presence of WWH produced 28.1 g l-1of PHB resulting in very high product yield coefficient of 0.94 g PHB per g oil. Since PHB yields were about 40 % higher than in the control cultivation, it seems that WWH can be considered as an excellent inexpensive nitrogen source for PHB production by C. necator.

Keywords

Whole whey hydrolyzate, Cupriavidus necator, poly(3-hydroxybutyrate), complex nitrogen source, waste frying oil

Authors

OBRUČA, S.; BENEŠOVÁ, P.; OBORNÁ, J.; MÁROVÁ, I.

RIV year

2014

Released

1. 3. 2014

Publisher

Springer

Location

Nizozemí

ISBN

0141-5492

Periodical

Biotechnology Letters

Year of study

Number

State

Kingdom of the Netherlands

Pages from

775

Pages to

781

Pages count

BibTex

@article{BUT105505,
  author="Stanislav {Obruča} and Pavla {Benešová} and Jana {Oborná} and Ivana {Márová}",
  title="Application of protease-hydrolyzed whey as a complex nitrogen source to increase poly(3-hydroxybutyrate) production from oils by Cupriavidus necator",
  journal="Biotechnology Letters",
  year="2014",
  volume="36",
  number="4",
  pages="775--781",
  doi="10.1007/s10529-013-1407-z",
  issn="0141-5492"
}