Rhodospirillum rubrum is bacterium with great ecological as well as biotechnological importance. This project aims at exploring role of PHA in context of its biological importance under various metabolic situations and conditions with insight into possible biotechnological consequences and applications.

Keywords
polyhydroxyalkanoates; PHA cycle; Rhodospirillum rubrum ; stress robustness

GF21-15958L

English

Obruča Stanislav, prof. Ing., Ph.D. - principal person responsible
Sedlář Karel, doc. Mgr. Ing., Ph.D. - fellow researcher

Faculty of Chemistry
- responsible department (2.4.2020 - 2.4.2020)
Institute of Food Science and Biotechnology
- responsible department (2.4.2020 - not assigned)
Department of Biomedical Engineering
- co-beneficiary (1.1.2021 - 31.12.2023)
Institute of Food Science and Biotechnology
- beneficiary (1.1.2021 - 31.12.2023)

JUREČKOVÁ, K.; NYKRÝNOVÁ, M.; SLANINOVÁ, E.; FLEURIOT-BLITMAN, H.; AMSTUTZ, V.; HEŘMÁNKOVÁ, K.; BEZDÍČEK, M.; MRÁZOVÁ, K.; HRUBANOVÁ, K.; ZINN, M.; OBRUČA, S.; SEDLÁŘ, K. Cultivation driven transcriptomic changes in the wild-type and mutant strains of Rhodospirillum rubrum. Computational and Structural Biotechnology Journal, 2024, vol. 23, no. December 2024, p. 2681-2694. ISSN: 2001-0370.
Detail

PACASOVÁ, V.; MRÁZOVÁ, K.; SLANINOVÁ, E.; OBRUČA, S. PHA Metabolism and Stress Response in Bacterium Rhodospirillum rubrum. 2023. p. 119-119.
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JUREČKOVÁ, K.; SLANINOVÁ, E.; OBRUČA, S.; FLEURIOT-BLITMAN, H.; ZINN, M.; SEDLÁŘ, K. Differential expression analysis of RNA-Seq data for identification of housekeeping genes in Rhodospirillum rubrum DSM 467. 11th European Symposium on Biopolymer (ESBP). 2023.
Detail

FLEURIOT-BLITMAN, H.; AMSTUTZ, V.; NYKRÝNOVÁ, M.; SLANINOVÁ, E.; SEDLÁŘ, K.; SEDLÁČEK, P.; OBRUČA, S.; HATZIMANIKATIS, V.; ZINN, M. Investigating the Relationship Between Respiratory Chain Activity and the Electron Sink Mechanism of Poly(3-hydroxybutyrate) (PHB) Accumulation in Rhodospirillum rubrum. 11th European Symposium on Biopolymer (ESBP). 2023.
Detail

NYKRÝNOVÁ, M.; JUREČKOVÁ, K.; MUSILOVÁ, J.; SLANINOVÁ, E.; OBRUČA, S.; FLEURIOT-BLITMAN, H.; ZINN, M.; SEDLÁŘ, K. Transcriptome Analysis of Rhodospirillum rubrum - Wild Type and Mutant Strains. 11th European Symposium on Biopolymer (ESBP). 2023.
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SLANINOVÁ, E.; JANOŠEC, J.; SEDLÁČEK, P.; OBRUČA, S. In situ study of pigments in metabolically versatile bacterium Rhodospirillum rubrum. Book of abstracts of the 10th International Conference on Chemical Technology. 1. Praha: AMCA,spol. s.r.o., 2023. p. 43-43. ISBN: 978-80-88214-40-3.
Detail

SLANINOVÁ, E.; OBRUČA, S.; KOCHERBITOV, V.; SEDLÁČEK, P. On the bioprotective effects of 3-hydroxybutyrate: Thermodynamic study of binary 3HB-water systems. BIOPHYSICAL JOURNAL, 2023, vol. 122, no. 3, p. 460-469. ISSN: 0006-3495.
Detail

JUREČKOVÁ, K.; NYKRÝNOVÁ, M.; SLANINOVÁ, E.; BEZDÍČEK, M.; OBRUČA, S.; ŠKUTKOVÁ, H.; SEDLÁŘ, K. Genomic and functional analysis of polyhydroxyalkanoates producer Rhodospirillum rubrum DSM 467. In Proceedings of the 9th International Conference on Chemical Technology. Prague: Czech Society of Industrial Chemistry, 2022. p. 367-372. ISBN: 978-80-88307-11-2.
Detail

FLEURIOT-BLITMAN, H.; AMSTUTZ, V.; NYKRÝNOVÁ, M.; SLANINOVÁ, E.; SEDLÁŘ, K.; SEDLÁČEK, P.; OBRUČA, S.; HATZIMANIKATIS, V.; ZINN, M. Increased CO2 gas-liquid mass transfer interferes with Rhodospirillum rubrum’s physiology and production of poly-3-hydroxybutyrate. 18th International Symposium on Biopolymers (ISBP). 2022.
Detail

SEDLÁŘ, K.; MUSILOVÁ, J.; ŠKUTKOVÁ, H.; NYKRÝNOVÁ, M.; JUREČKOVÁ, K.; PERNICOVÁ, I.; SLANINOVÁ, E.; FLEURIOT-BLITMAN, H.; ZINN, M.; OBRUČA, S. Improving structural annotation of Rhodospirillum rubrum DSM 467 with RNA-Seq. 18th International Symposium on Biopolymers (ISBP). 2022.
Detail

NYKRÝNOVÁ, M.; JUREČKOVÁ, K.; ŠKUTKOVÁ, H.; SLANINOVÁ, E.; OBRUČA, S.; FLEURIOT-BLITMAN, H.; ZINN, M.; SEDLÁŘ, K. Gene expression profiling in Rhodospirillum rubrum. 18th International Symposium on Biopolymers (ISBP). 2022.
Detail

SLANINOVÁ, E.; MRÁZOVÁ, K.; HAVLÍKOVÁ, M.; SEDLÁČEK, P.; ZINN, M.; OBRUČA, S. Rhodospirillum rubrum in the spotlight - use of advanced microscopy techniques for analysis of PHA accumulating microorganisms. ISBP 2022,18th Int´l Symposium on Biopolymers, Sion - Switzerland, 13th-16th September 2022. 1. Švýcarsko: HES-SO Valais-Wallis, 2022. p. 129-129.
Detail

SLANINOVÁ, E.; RUBANOVÁ, B.; ČERNAYOVÁ, D.; NOVÁČKOVÁ, I.; HAVLÍKOVÁ, M.; MRÁZOVÁ, K.; SEDLÁČEK, P.; OBRUČA, S. Study of aeration and light/dark conditions on production of polyhydroxyalkanoates in bacteria Rhodospirillium rubrum. 2022. p. 1 (1 s.).
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Responsibility: Obruča Stanislav, prof. Ing., Ph.D.