Project - Directed evolution of polyhydroxyalkanoates producing bacteria

Funding resources

Czech Science Foundation - Standardní projekty

- whole funder (2019-01-01 - 2021-12-31)

On the project

The main goal of the project is to perform the evolutionary engineering of PHAs producing bacteria in order to better understand adaptive mechanisms of industrially relevant bacteria and to explore the role of PHAs in their adaptation to adverse conditions. Further, our aim is also to obtain robust bacterial strains resistant to the most important stress factors associated with anthropogenic and/or environmental stressors for in situ bioremediation as well as PHA producers adapted to microbial inhibitors present in lignocellulose-based substrates. To reach these goals, highly multidisciplinary approach combining various fields of science such as biochemistry, molecular biology, biophysics and also advanced techniques of microspectroscopy and cell manipulation will be applied.

Keywords
evolutionary engineering; PHA; bacteria; stress response; adaptation

Mark

GA19-20697S

Default language

English

People responsible

Sedláček Petr, doc. Ing., Ph.D. - fellow researcher
Obruča Stanislav, prof. Ing., Ph.D. - principal person responsible

Units

Materials Research Centre
- beneficiary (2018-04-03 - not assigned)

Results

NOVÁČKOVÁ, I.; KUČERA, D.; POŘÍZKA, J.; PERNICOVÁ, I.; SEDLÁČEK, P.; KOLLER, M.; KOVALČÍK, A.; OBRUČA, S. Adaptation of cupriavidus necator to levulinic acid for enhanced production of P(3HB- co -3HV) copolyesters. BIOCHEMICAL ENGINEERING JOURNAL, 2019, vol. 151, no. 11, p. 1-10. ISSN: 1369-703X.
Detail

MÜLLEROVÁ, L.; MARKOVÁ, K.; MRAVEC, F.; OBRUČA, S. Use of Flavin-Related Cellular Autofluorescence to Monitor Processes in Microbial Biotechnology. Microorganisms, 2022, vol. 10, no. 6, p. 1-21. ISSN: 2076-2607.
Detail

KUČERA, D.; NOVÁČKOVÁ, I.; PERNICOVÁ, I.; SEDLÁČEK, P.; OBRUČA, S. Biotechnological Production of Poly(3-Hydroxybutyrate-co-4-Hydroxybutyrate-co-3-Hydroxyvalerate) Terpolymer by Cupriavidus sp. DSM 19379. Bioengineering, 2019, vol. 6, no. 3, p. 74-84. ISSN: 2306-5354.
Detail

PERNICOVÁ, I.; NOVÁČKOVÁ, I.; KOUŘILOVÁ, X.; KUČERA, D.; OBRUČA, S. Isolation extremopilic bacteria producing polyhydroxyalkanoate from natural sample. The biomania student scientific meeting and eusynbios symposium 2019 Book of abstracts. Brno, Czech Republic: Masaryk University Press, 2019. p. 114-114. ISBN: 978-80-210-9373-7.
Detail

PERNICOVÁ, I.; NOVÁČKOVÁ, I.; KOUŘILOVÁ, X.; HERNÁNDEZ, N.; PRIETO, M.A.; OBRUČA, S. Thermophilic bacteria isolated from compost as producers of polyhydroxyalkanoates. 10th European Symposium on Biopolymers. Straubing, Germany: C.A.R.M.E.N.e.V., 2019. p. 72-72.
Detail

NOVÁČKOVÁ, I.; KUČERA, D.; OBRUČA, S.; SEDLÁČEK, P.; POŘÍZKA, J.; PERNICOVÁ, I. Adaptation of PHA producing bacteria Cupriavidus necator H16 and Halomonas halophila to biotechnologically relevant stressors. 10th European Symposium on Biopolymers - Program. 2019. p. 70-70.
Detail

NOVÁČKOVÁ, I.; KUČERA, D.; POŘÍZKA, J.; PERNICOVÁ, I.; SEDLÁČEK, P.; OBRUČA, S. Application of evolutionary engineering for enhancement of robustness and producing capacity of selected microbial PHA producers. The Biomania student scientific meeting and Eusynbios symposium 2019 - Book of Abstracts. 1. 2019. p. 120-120. ISBN: 978-80-210-9373-7.
Detail

NOVÁČKOVÁ, I.; KUČERA, D.; POŘÍZKA, J.; SEDLÁČEK, P.; PERNICOVÁ, I.; OBRUČA, S. Evolutionary engineering approach for enhancement of growth characteristics and producing capacity of selected PHA producing microorganisms. Studentská odborná konference Chemie je život 2019 - Sborník abstraktů. Brno: Vysoké učení technické v Brně, Fakulta chemická, 2019. p. 58-58. ISBN: 978-80-214-5807-9.
Detail

PERNICOVÁ, I.; NOVÁČKOVÁ, I.; SEDLÁČEK, P.; KOUŘILOVÁ, X.; KOLLER, M.; OBRUČA, S. Application of osmotic challenge for enrichment of microbial consortia in polyhydroxyalkanoates producing thermophilic and thermotolerant bacteria and their subsequent isolation. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2020, vol. 144, no. 2, p. 698-704. ISSN: 0141-8130.
Detail

OBRUČA, S.; SEDLÁČEK, P.; SLANINOVÁ, E.; FRITZ, I.; DAFFERT, C.; MEIXNER, K.; ŠEDRLOVÁ, Z.; KOLLER, M. Novel unexpected functions of PHA granules. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2020, vol. 104, no. 11, p. 4795-4810. ISSN: 0175-7598.
Detail

PERNICOVÁ, I.; NOVÁČKOVÁ, I.; SEDLÁČEK, P.; KOUŘILOVÁ, X.; KALINA, M.; KOVALČÍK, A.; KOLLER, M.; NEBESAROVA, J.; KRZYZANEK, V.; HRUBANOVA, K.; MÁSILKO, J.; SLANINOVÁ, E.; OBRUČA, S. Introducing the Newly Isolated BacteriumAneurinibacillussp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers-1. Isolation and Characterization of the Bacterium. Polymers, 2020, vol. 12, no. 6, p. 1-13. ISSN: 2073-4360.
Detail

SEDLÁČEK, P.; PERNICOVÁ, I.; NOVÁČKOVÁ, I.; KOUŘILOVÁ, X.; KALINA, M.; KOVALČÍK, A.; KOLLER, M.; NEBESAROVA, J.; KRZYZANEK, V.; HRUBANOVA, K.; MÁSILKO, J.; SLANINOVÁ, E.; TRUDIČOVÁ, M.; OBRUČA, S. Introducing the Newly Isolated BacteriumAneurinibacillussp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers-2. Material Study on the Produced Copolymers. Polymers, 2020, vol. 12, no. 6, p. 1-18. ISSN: 2073-4360.
Detail

KOUŘILOVÁ, X.; PERNICOVÁ, I.; SEDLÁŘ, K.; MUSILOVÁ, J.; SEDLÁČEK, P.; KALINA, M.; KOLLER, M.; OBRUČA, S. Production of polyhydroxyalkanoates (PHA) by a thermophilic strain of Schlegelella thermodepolymerans from xylose rich substrates. BIORESOURCE TECHNOLOGY, 2020, vol. 315, no. 8, p. 1-7. ISSN: 0960-8524.
Detail

Zdeněk Pilát1*, Alexandr Jonáš1, Jana Pilátová2, Tereza Klementová1, Silvie Bernatová1, Martin Šiler1, Tadeáš Maňka1, Martin Kizovský1, Filip Růžička3, Roman Pantůček4, Ute Neugebauer5,6, Ota Samek1, Pavel Zemánek. Analysis of Bacteriophage-Host Interaction by Raman Tweezers. Analytical Chemistry, 2020, vol. 92, no. 18, p. 12304-12311. ISSN: 1520-6882.
Detail

CHATRNÁ, V.; NOVÁČKOVÁ, I.; OBRUČA, S. Characterization of Bacterial Strains Obtained in Evolutionary Engineering. Studentská odborná konference CHEMIE JE ŽIVOT 2020 Sborník abstraktů. Brno: Vysoké učení technické v Brně, Fakulta chemická, 2020. p. 75-76. ISBN: 978-80-214-5920-0.
Detail

PERNICOVÁ, I.; KUČERA, D.; NEBESÁŘOVÁ, J.; KALINA, M.; NOVÁČKOVÁ, I.; KOLLER, M.; OBRUČA, S. Production of polyhydroxyalkanoates on waste frying oil employing selected Halomonas strains. BIORESOURCE TECHNOLOGY, 2019, vol. 292, no. 11, p. 1-4. ISSN: 0960-8524.
Detail

OBRUČA, S.; SEDLÁČEK, P.; PERNICOVÁ, I.; KOVALČÍK, A.; NOVÁČKOVÁ, I.; SLANINOVÁ, E.; MÁROVÁ, I. Interconnection between PHA and Stress Robustness of Bacteria. In The Handbook of Polyhydroxyalkanoates. 1. Boca Raton: CRC Press, 2020. p. 107-132. ISBN: 9780367275594.
Detail

OBRUČA, S.; SEDLÁČEK, P.; KOLLER, M. The underexplored role of diverse stress factors in microbial biopolymer synthesis. BIORESOURCE TECHNOLOGY, 2021, vol. 326, no. 4, p. 1-12. ISSN: 0960-8524.
Detail

KOUŘILOVÁ, X.; NOVÁČKOVÁ, I.; KOLLER, M.; OBRUČA, S. Evaluation of mesophilic Burkholderia sacchari, thermophilic Schlegelella thermodepolymerans and halophilic Halomonas halophila for polyhydroxyalkanoates production on model media mimicking lignocellulose hydrolysates. BIORESOURCE TECHNOLOGY, 2021, vol. 325, no. 4, p. 1-5. ISSN: 0960-8524.
Detail

KOUŘILOVÁ, X.; SCHWARZEROVÁ, J.; PERNICOVÁ, I.; SEDLÁŘ, K.; MRÁZOVÁ, K.; KRZYŽÁNEK, V.; NEBESÁŘOVÁ, J.; OBRUČA, S. The First Insight into Polyhydroxyalkanoates Accumulation in Multi-Extremophilic Rubrobacter xylanophilus and Rubrobacter spartanus. Microorganisms, 2021, vol. 9, no. 5, p. 1-13. ISSN: 2076-2607.
Detail

SLANINOVÁ, E.; SEDLÁČEK, P.; NEBESÁŘOVÁ, J.; KRZYŽÁNEK, V.; SAMEK, O.; OBRUČA, S. Application of advanced microscopy in revealing biological role and application potential of bacterial polyhydroxyalkanoates. Vídeňská 1083,14220,Prague 4: Czech-BioImaging, 2021. p. 18-18.
Detail

NOVÁČKOVÁ, I.; HRABALOVÁ, V.; SLANINOVÁ, E.; SEDLÁČEK, P.; POŘÍZKA, J.; KALINA, M.; SAMEK, O.; OBRUČA, S. Evolutionary Engineering of Selected Polyhydroxyalkanoate Producing Strains to Biotechnologically Relevant Stressors. World Academy of Science, Engineering and Technology. 2021. p. 76-76. ISSN: 1307-6892.
Detail

HEŘMÁNKOVÁ, K.; KOUŘILOVÁ, X.; PERNICOVÁ, I.; BEZDÍČEK, M.; LENGEROVÁ, M.; OBRUČA, S.; SEDLÁŘ, K. Complete Genome Sequence of the Type Strain Tepidimonas taiwanensis LMG 22826T, a Thermophilic Alkaline Protease and Polyhydroxyalkanoate Producer. GENOME BIOL EVOL, 2021, vol. 13, no. 12, p. 1-5. ISSN: 1759-6653.
Detail

KOUŘILOVÁ, X.; PERNICOVÁ, I.; VIDLÁKOVÁ, M.; KREJČIŘÍK, R.; MRÁZOVÁ, K.; HRUBANOVÁ, K.; KRZYŽÁNEK, V.; NEBESÁŘOVÁ, J.; OBRUČA, S. Biotechnological Conversion of Grape Pomace to Poly(3-hydroxybutyrate) by Moderately Thermophilic Bacterium Tepidimonas taiwanensis. Bioengineering, 2021, vol. 8, no. 10, p. 1-13. ISSN: 2306-5354.
Detail

OBRUČA, S.; DVOŘÁK, P.; SEDLÁČEK, P.; KOLLER, M.; SEDLÁŘ, K.; PERNICOVÁ, I.; ŠAFRÁNEK, D. Polyhydroxyalkanoates synthesis by halophiles and thermophiles: towards sustainable production of microbial bioplastics. BIOTECHNOLOGY ADVANCES, 2022, vol. 58, no. 9, p. 1-28. ISSN: 0734-9750.
Detail

NOVÁČKOVÁ, I.; HRABALOVÁ, V.; SLANINOVÁ, E.; SEDLÁČEK, P.; SAMEK, O.; KOLLER, M.; KRZYZANEK, V.; HRUBANOVÁ, K.; MRÁZOVÁ, K.; NEBESÁŘOVÁ, J.; OBRUČA, S. The role of polyhydroxyalkanoates in adaptation of Cupriavidus necator to osmotic pressure and high concentration of copper ions. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2022, vol. 206, no. 5, p. 977-189. ISSN: 0141-8130.
Detail

OBRUČA, S.; PERNICOVÁ, I.; KUČERA, D.; NOVÁČKOVÁ, I.; SEDLÁČEK, P.; Vysoké učení technické v Brně, Brno, CZ: Způsob výroby polyhydroxyalkanoátů pomocí isolátu termofilního bakteriálního kmene Aneurinibacillus sp. H1. 308626, patent. (2021)
Detail

Responsibility: Obruča Stanislav, prof. Ing., Ph.D.