Project detail
The development of encapsulation magnetic nano- and microparticles using real time polymerase chain reaction
Duration: 01.02.2013 — 24.11.2016
Funding resources
Czech Science Foundation - Postdoktorandské granty
- whole funder (2013-02-01 - 2016-11-24)
On the project
Navrhovaný projekt se zabývá vývojem jednoduché metody pro studium enkapsulace magnetických jader pri syntéze nano- a mikrocástic pro aplikace v molekulární diagnostice. Oxidy železa a nekteré látky používané pri stabilizaci magnetických jader snižují biokompatibilitu navrhovaných nosicu, proto je dokonalé pokrytí jader duležitým predpokladem pro jejich použití v molekulární diagnostice. Stupen pokrytí nosicu ruznými nízko- nebo vysokomolekulárními látkami (polymerní vrstvou) bude overován pomocí polymerázové retezové reakce v reálném case (RT-PCR). V závislosti na dokonalosti pokrytí nosicu výše uvedenými látkami bude sledována úcinnost amplifikace DNA. Výsledky budou statisticky vyhodnoceny. Konecným cílem projektu bude príprava magnetických nano- a mikrocástic vhodných pro molekulárne diagnostické aplikace.
Description in English
The proposed project deals with the development of simple method for the study of magnetic cores encapsulation in the
synthesis of nano-and microparticles for applications in molecular diagnostics. Iron oxides and some substances used to
stabilize magnetic cores reduce biocompatibility of the proposed carriers. Therefore perfectly encapsulated cores are very
important for their application in molecular diagnostics. The degree of carrier’s coverage with a variety of low-or highmolecular
weight compounds (polymer layer) will be verified by polymerase chain reaction in real time (RT-PCR). The
effectiveness of DNA amplification will be followed up depending on the carrier’s encapsulation by the above mentioned agents.
The results will be statistically evaluated. Final aim of the project is to prepare magnetic nano-and microparticles suitable for
molecular diagnostic applications.
Keywords
enkapsulace magnetických částic, PCR v reálném čase
Key words in English
magnetic particles encapsulation, real-time polymerase chain reaction
Mark
GP13-32840P
Default language
Czech
People responsible
Trachtová Štěpánka, Ing., Ph.D. - principal person responsible
Units
Faculty of Chemistry
- beneficiary (2013-02-19 - not assigned)
Results
TRACHTOVÁ, Š.; ŠPANOVÁ, A.; PRETTL, Z.; HORÁK, D.; RITTICH, B. Magnetic driven separation techniques - DNA isolation from probiotic food samples. Microbiology and Immunology of Mucosa Probiotics Conference 2013. 1. Kudowa Zdrój, Polsko: Institute of Immunology and Experimental Therapy Polish Academy of Science, 2013. p. 27-27. ISBN: 978-83-928488-3-7.
Detail
TRACHTOVÁ, Š.; ŠPANOVÁ, A.; TÓTH, J.; PRETTL, Z.; HORÁK, D.; RITTICH, B.; GYENIS, J. Magnetic driven separation techniques. Kudowa Zdrój, Polsko: Institute of Immunology and experimental therapy Polish Academy of Science, 2013. p. 27-27.
Detail
ČAKAJDOVÁ, M.; TRACHTOVÁ, Š.; ŠPANOVÁ, A.; HORÁK, D.; RITTICH, B. DNA based identification of microorganisms in a probiotic products. Microbiology and Immunology of Mucosa Probiotics Conference 2013. 1. Kudowa Zdrój, Polsko: Institute of Immunology and Experimental Therapy Polish academy of Science, 2013. p. 41-41. ISBN: 978-83-928488-3-7.
Detail
TRACHTOVÁ, Š.; ŠPANOVÁ, A.; HORÁK, D.; RITTICH, B. The Study of magnetic P(HEMA-co-GMA) and P(GMA) microspheres using real-time PCR. Frontiers of Polymer Colloids: From Synthesis to Macro-Scale and Nano-Scale Applications - Programme Booklet. 1. Praha, Czech Republic: Institute of Macromolecular Chemistry, Academy Science of the Czech Republic, 2014. p. 145-145. ISBN: 978-80-85009-80-4.
Detail
TRACHTOVÁ, Š.; ŠPANOVÁ, A.; HORÁK, D.; RITTICH, B.; ZAPLETALOVÁ, H.; KOLÁŘOVÁ, H. The study of encapsulation magnetic nano- and microparticles using real time polymerase chain reaction. BOOK OF ABSTRACT CEITEC Annual Conference “Frontiers in Material and Life Sciences. Czech Republic, Brno: Masaryk University, Brno, 2014. p. 233-233. ISBN: 978-80-210-7159-9.
Detail
TRACHTOVÁ, Š.; ŠPANOVÁ, A.; RITTICH, B.; HORÁK, D. Study on the encapsulation of magnetic cores using real time polymerase chain reaction. Dresden, Germany: Technische Universitat Dresden, 2014. p. 166-166.
Detail
TRACHTOVÁ, Š.; ŠPANOVÁ, A.; HORÁK, D.; HLÍDKOVÁ, H.; KOSTIV, U.; PATSULA, V.; ZAPLETALOVÁ, H.; RITTICH, B. The evaluation of magnetic particles suitable for DNA isolation using real time PCR. Bielawa, Polsko: Institute of Immunology and Experimental Therapy Polish Academy of Science, 2015.
Detail
TRACHTOVÁ, Š.; ŠPANOVÁ, A.; TÓTH, J.; PRETTL, Z.; HORÁK, D.; GYENIS, J.; RITTICH, B. Solid-phase DNA isolation from food matrices using hydrophilic magnetic microspheres. FOOD AND BIOPRODUCTS PROCESSING, 2015, vol. 94, no. 1, p. 375-381. ISSN: 0960-3085.
Detail
HORÁK, D.; HLÍDKOVÁ, H.; TRACHTOVÁ, Š.; ŠLOUF, M.; RITTICH, B.; ŠPANOVÁ, A. Evaluation of poly(ethylene glycol)-coated monodispersed magnetic poly(2-hydroxyethyl methacrylate) and poly(glycidyl methacrylate) microspheres by PCR. European Polymer Journal, 2015, vol. 68, no. 1, p. 687-696. ISSN: 0014-3057.
Detail
TRACHTOVÁ, Š.; ZAPLETALOVÁ, H.; ŠPANOVÁ, A.; HORÁK, D.; KOLÁŘOVÁ, H.; RITTICH, B. The evaluation of magnetic polymethacrylate-based microspheres used for solid phase DNA micro-extraction. Chromatography, 2015, vol. 2, no. 2, p. 156-166. ISSN: 2227-9075.
Detail
TRACHTOVÁ, Š.; ŠPANOVÁ, A.; HORÁK, D.; HLÍDKOVÁ, H.; KOSTIV, U.; RITTICH, B. The study of biocompatibility of magnetic particles using amplification methods. Brno, Czech Republic: 2015. p. 168-168.
Detail
TRACHTOVÁ, Š.; ŠPANOVÁ, A.; HORÁK, D.; KOZÁKOVÁ, H.; RITTICH, B. Real-time polymerase chain reaction as a tool for evaluation of magnetic poly(glycidyl methacrylate)-based microspheres in molecular diagnostics. CURRENT PHARMACEUTICAL DESIGN, 2016, vol. 22, no. 5, p. 639-646. ISSN: 1381-6128.
Detail
VAŠÍČEK, R.; VAŇÁSEK, J.; KONEČNÁ, J.; TRACHTOVÁ, Š.; ŠPANOVÁ, A.; RITTICH, B. Detection of Lactobacillus acidophilus species in the probiotic meat product. Brno, Czech Republic: Brno University of Technology, Faculty of Chemistry, 2015. p. 103-103.
Detail
VAŠÍČEK, R.; VAŇÁSEK, J.; KONEČNÁ, J.; TRACHTOVÁ, Š.; ŠPANOVÁ, A.; RITTICH, B. Detection of Lactobacillus acidophilus species in the probiotic meat product. Chemistry & Life 2015 – Book of Abstracts. 1. Brno, Czech Republic: Brno University of Technology, Faculty of Chemistry, 2015. p. 103-103. ISBN: 978-214-5228-2.
Detail
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