Publication detail

The study of encapsulation magnetic nano- and microparticles using real time polymerase chain reaction

TRACHTOVÁ, Š. ŠPANOVÁ, A. HORÁK, D. RITTICH, B. ZAPLETALOVÁ, H. KOLÁŘOVÁ, H.

Original Title

Type

abstract

Language

English

Original Abstract

Nano- and microparticles for biotechnology applications usually consist of a number of components: superparamagnetic core, protective coating and the surface functionality. Magnetic cores are covered with a variety of functionalized low-or high-molecular compounds (polymer layer). Iron oxides and some substances used to stabilize magnetic cores can reduce biocompatibility of the proposed particles; therefore perfectly encapsulated cores are very important for their application. The aim of work was the development of simple method for the study of magnetic cores encapsulation of nano- and microparticles suitable for applications in molecular diagnostics. The degree of magnetic particles coverage with a variety of low-or high molecular weight compounds (polymer layer) was verified by real time polymerase chain reaction (qPCR). The proposed method is based on estimation of DNA amplification effectiveness in qPCR. Serial dilutions of different types of magnetic particles, such as (hydrophilic non-porous poly (2-hydroxyethyl methacrylate-co-glycidyl methacrylate) P(HEMA-co-GMA) and poly(glycidy lmethacrylate) P(GMA), poly[(glycidyl methacrylate)-co-[2-(methacryloyloxy)ethoxy]acetic-co-ethylene dimethacrylate]P(GMA-MOEAA)-PEG, poly[(2-hydroxyethyl methacrylate)-co-[2-(methacryloyloxy)ethoxy]acetic-co-ethylene dimethacrylate] P(HEMA-MOEAA)-PEG and some individual components used for their preparation were tested. The results of study have shown that the real-time PCR can be used for the study of characteristics of magnetic particles according to their compatibility with DNA amplification.

Keywords

real-time PCR, magnetic particles encapsulation

Authors

TRACHTOVÁ, Š.; ŠPANOVÁ, A.; HORÁK, D.; RITTICH, B.; ZAPLETALOVÁ, H.; KOLÁŘOVÁ, H.

Released

21. 10. 2014

Publisher

Masaryk University, Brno

Location

Czech Republic, Brno

ISBN

978-80-210-7159-9

Book

BOOK OF ABSTRACT CEITEC Annual Conference “Frontiers in Material and Life Sciences

Pages from

233

Pages to

233

Pages count

BibTex

@misc{BUT110225,
  author="Štěpánka {Trachtová} and Alena {Španová} and Daniel {Horák} and Bohuslav {Rittich} and Hana {Zapletalová} and Hana {Kolářová}",
  title="The study of encapsulation magnetic nano- and microparticles using real time polymerase chain reaction",
  booktitle="BOOK OF ABSTRACT CEITEC Annual Conference “Frontiers in Material and Life Sciences",
  year="2014",
  pages="233--233",
  publisher="Masaryk University, Brno",
  address="Czech Republic, Brno",
  isbn="978-80-210-7159-9",
  note="abstract"
}