Course detail
Laboratory Classes in Molecular Biotechnology
FCH-MC_MOB_PAcad. year: 2009/2010
Safety of work in microbiological and biotechnological laboratory. Arrangement of PCR laboratory. Cultivation of Lactobacillus acidophilus cells for isolation of chromosomal DNA. Cell lysis. Removal of proteins using phenol extraction. Precipitation of DNA with ethanol. Verification of DNA integrity using agarose gel electrophoresis. Spectrophotometric and fluorometric estimation of concentration and purity verification of DNA. Preparation of PCR mixture for amplification of Lactobacillus acidophilus using purified DNA as DNA matrix. Negative controls. PCR amplification. Detection of PCR products using agarose gel electrophoresis. Estimation of length of amplified fragment using external DNA standards. Sensitivity of PCR. Isolation of DNA from dairy product (yoghurt) using magnetic particles. Identification of Lactobacillus DNA in dairy product (yoghurt) by PCR.Identification of Lactobacillus DNA in dairy product (yoghurt) by real time PCR. Test - identifikation of Lactobacillus cells in unknown sample using PCR.
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Planned learning activities and teaching methods
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Aims
Specification of controlled education, way of implementation and compensation for absences
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Basic literature
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Classification of course in study plans
- Programme NPCP_CHTP Master's
branch NPCO_CHTP , 1 year of study, winter semester, compulsory-optional
branch NPCO_CHTP , 2 year of study, winter semester, compulsory-optional - Programme NKCP_CHTP Master's
branch NKCO_CHTP , 1 year of study, winter semester, compulsory-optional
branch NKCO_CHTP , 2 year of study, winter semester, compulsory-optional - Programme CKCP_CZV lifelong learning
branch CKCO_CZV , 1 year of study, winter semester, compulsory-optional
Type of course unit
Laboratory exercise
Teacher / Lecturer