Course detail

Bioengineering II

FCH-MC_BIO2Acad. year: 2009/2010

Stechiometry of microbial growth and material balances. Systems with immobilized cells and enzymes. Enzymatic catalysis in anhydrous and quasi anhydrous solvents. Magnetic particles. Micromanipulation of particles. Microflow in capillary tubes. Microdrops. Properties of fluids. Mechanical, chemical and enzymatic cell disruption. Precipitation and salting-out odf proteins. Filtration. Centrifugation. Extraction - types of extractors and their design. Extraction using aqueous two-phase systems. Large scale chromatographic techniques. Production of proteins using genetically modified microorganisms (GMO) and methods of their isolation. Storage of proteins. Validation of production quality.

Language of instruction

Czech

Number of ECTS credits

Mode of study

Not applicable.

Guarantor

doc. Ing. Bohuslav Rittich, CSc.

Department

Institute of Food Science and Biotechnology (ÚCHPBT)

Learning outcomes of the course unit

Theoretical aspects of various isolation and separation techniques focused on microorganism cells

Prerequisites

Without restriction

Co-requisites

Not applicable.

Planned learning activities and teaching methods

Teaching methods depend on the type of course unit as specified in the article 7 of BUT Rules for Studies and Examinations.

Assesment methods and criteria linked to learning outcomes

Oral examination

Course curriculum

1. Stechiometry of microbial growth and material balances.
2. Systems with immobilized cells and enzymes.
3. Enzymatic catalysis in anhydrous and quasi anhydrous solvents.
4. Magnetic particles. Micromanipulation of particles.
5. Microflow in capillary tubes. Microdrops. Properties of fluids.
6. Mechanical, chemical and enzymatic cell disruption.
7. Precipitation and salting-out odf proteins. Filtration. Centrifugation.
8. Extraction - types of extractors and their design.
9. Extraction using aqueous two-phase systems.
10. Large scale chromatographic techniques.
11. Production of proteins using genetically modified microorganisms (GMO) and methods of their isolation.
12. Protein stability.
13. Validation of production quality.

Work placements

Not applicable.

Aims

Lectures cover methods for biotechnoly product processing

Specification of controlled education, way of implementation and compensation for absences

Attendance at lectures is not obligatory but it is recommended. At seminars the calculations of model examples are given

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Not applicable.

Type of course unit

Lecture

26 hod., optionally

Teacher / Lecturer

doc. Ing. Bohuslav Rittich, CSc.

Exercise

13 hod., compulsory

Teacher / Lecturer

Mgr. Kristýna Turková, Ph.D.
Mgr. Michala Kotianová
doc. Ing. Bohuslav Rittich, CSc.