Course detail
Advanced Fluorescence Techniques
FCH-DA_PFTAcad. year: 2025/2026
The course is designed as a practically focused seminar, which will enable students to acquaint themselves with advanced fluorescence techniques on a suitable model system. Theoretical and practical lessons will alternate within the seminar. The theoretical parts will focus on specific aspects of preparation, measurement and interpretation of the fluorescence techniques and will build on the self-study of the fundamental fundamentals of the given techniques. The practical part includes the preparation of the model system itself (associative colloid system), measurement and evaluation. Within the subject, the following techniques will be discussed: time-resolved emission spectroscopy, anisotropy, resonance energy transfer, fluorescence quenching, fluorescence correlation spectroscopy, two-color fluorescence cross-correlation spectroscopy. Together with the introductory lesson of these 6 themes with the theoretical and practical part will create the framework of the semester. At the end of the semester, the basics will be created to create a short monograph on the behavior of the selected system from the perspective of advanced fluorescence techniques.
Language of instruction
Mode of study
Guarantor
Entry knowledge
Rules for evaluation and completion of the course
To attend the course, participation in theoretical and practical lessons is required.
Aims
The output of the study is practical skills in advanced experimental methods of fluorescence spectroscopy. Students will learn the basics of techniques used in advanced research, will learn the basics of interpretation of such data and will verify this knowledge on a simple system.
Study aids
Prerequisites and corequisites
Basic literature
Valeur, B.; Berberan-Santos, M.N. Molecular Fluorescence: Principles and Applications, Wiley‐VCH Verlag GmbH & Co. KGaA, 2012 (EN)
Recommended reading