Course detail
Molecular Biotechnology
FCH-MC_MOBAcad. year: 2025/2026
The course is focused on the explanation of procedures used in molecular biotechnology for construction of genetically modified organisms with required characteristics. It explains the procedures used for gene transfer from one organism to the other with the aim to obtain an useful product including procedures used for optimalisation of gene expression and stability of recombinant proteins in procaryotic and eucaryotic cells. The impact of transgenic microorganisms, plants and animals in our life and their practical use are explained in addition to their construction.
Language of instruction
Number of ECTS credits
Mode of study
Guarantor
Entry knowledge
Rules for evaluation and completion of the course
Attendance at lectures is not obligatory but it is recommended
Aims
Theoretical aspects of various experimental techniques used in molecular biotechnology research in GMO construction and their practical use.
Study aids
Prerequisites and corequisites
Basic literature
Recommended reading
Glick B.R., Molecular biotechnology, American Society for Microbiology, 2017 (CS)
Classification of course in study plans
- Programme NPCP_CHMA Master's
specialization CHBL , 1 year of study, winter semester, compulsory-optional
- Programme NPCP_CHPL Master's 1 year of study, winter semester, compulsory-optional
- Programme NKCP_CHPL Master's 1 year of study, winter semester, compulsory-optional
- Programme NPCP_PCHBT Master's 1 year of study, winter semester, compulsory-optional
Type of course unit
Lecture
Teacher / Lecturer
Syllabus
924/5000
• The relationship between chemical production and biotechnology.
• DNA isolation and recombinant DNA technology.
• DNA cloning, polymerase chain reaction, PCR utilization in clinical and food diagnostics
• Cloning vectors and their use.
• Transformacfe - Transfer of foreign DNA into cells. Selection of cloned gene cells.
• Fusion proteins. Translational expression vectors. Enhancement of protein stability. Integration and shuttle vectors.
• Yeast expression systems. Systems using insect cells, mammalian expression systems.
• Biotechnological use of genetically modified micro-organisms.
• Transgenic plants and animal animals.
• Bioinformatics in gene biotechnology
• Use of bioinformatic approaches in the study of nucleic acids and proteins
• Gene therapy Diseases treatable by gene therapy. Vectors for gene therapy. Perspectives of gene therapy.
• Development, characterization and antibody production
Guided consultation in combined form of studies
Teacher / Lecturer