Course detail

Analytical Chemistry II

FCH-BC_ANC2Acad. year: 2025/2026

This course aims to introductuion students to the basic methods and techniques of instrumental analytical chemistry. Teaching is structured in the following thematic areas: Physical aspects of analytical methods. Analytical signal and its qulitative and quantitative evaluation. Principles of selected instrumental methods-spectrometric and electroanalytical methods, their qualitative and quantitative aspects and practical examples. Potentiometry. Voltametry and polarography (classical, pulse, stripping). Titrations with polarizable electrodes. Electrogavimetry, coulometry. Conductometry, dielectrimetry. Optical methods, their classification, properties of electromagnetic radiation. Refractometry, interferometry, polarimetry, nephelometry, turbidimetry. Electronic absorption spectra, instrumentation. UV-VIS molecular spectrophotometry. Luinescence methods: molecular fluorescence, phosphorescence, chemiluminescence, bioluminescence. Absorption and emission atomic spectrometry. Principles and importance of chromatographic and electromigration methods. Introduction to analytical chemometry. Methods of quantitative evaluation.

Language of instruction

Czech

Number of ECTS credits

Mode of study

Not applicable.

Guarantor

doc. Mgr. Michaela Vašinová Galiová, Ph.D.

Department

Institute of Chemistry and Technology of Environmental Protection (ÚCHTOŽP)

Entry knowledge

Knowledge from the course Analytical chemistry I, knowledge from physics (electricity, magnetism), knowledge from physical chemistry

Rules for evaluation and completion of the course

Course credit is awarded for 100% attendance at seminars (any absence must be properly apologized). Credit may be obtained for successful completing of three partial written tests during the semester (ie getting at least 6 points out of 10 possible). In case of failure in one partial test (or more) course credit could be awarded for the successful completing of the final test (ie, obtaining at least 60 points out of 100).
Condition for passing the exam is to obtain credit from the exercise of Analytical Chemistry II.
The exam is oral, evaluate the level of knowledge of theory and practice of analytical chemistry and creative approach to solving analytical problems.
Attendance at lectures is recommended, but not checked. Attendance at seminars is compulsory and is checked.
The students' work is controlled by three partial written tests during the semester in computational exercises.
For students in combined form of study are organized consultations on the topics for full-time students.
Consultations are also provided to full-time students on request.

Aims

The aim of the course is to provide students with an overview of selected instrumental analytical methods from the field of electrochemistry, spectrophotometry and separation techniques and to familiarize them with their application fields and their limitations.
By completion of the course the student will gain the following knowledge and skills:
1. They will know in depth the principles of simple instrumental analytical methods and possibilities of their application.
2. They will be able to select the optimal method for solving of specific analytical problems of analytical practice.
3. They will adopt the basic principles of chemometric treatment of analytical results obtained using simple instrumental-analytical methods.

Study aids

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Douglas A. Skoog et.al.: Analytická chemie, 2019 (CS)
K. Záruba a spol.: Analytická chemie 1. a 2. díl, 2016 (CS)
Kotouček M., Skopalová J.: Příklady z analytické chemie. Vydavatelství Univerzity Palackého Olomouc, 1997. (CS)

Type of course unit

Lecture

26 hod., optionally

Teacher / Lecturer

doc. Mgr. Michaela Vašinová Galiová, Ph.D.

Syllabus

1. Introduction to instrumental analysis. Position and importance of intrumental analytical techniques, their historical development, definition of basic concepts.

Topic I: Electroanalytical Methods
2. Potentiometry
3. Voltammetry and polarography (classical, differential pulse, dissolution, adsorption). Potentiometric dissolution analysis, titration with polarizable electrodes. Electrogravimetry, Coulometry, Conductometry.

Topic II: Spectrometry
4. Classification of spectral methods, basic physical principles, properties of electromagnetic radiation. Non-spectral methods: Refractometry, interferometry, polarimetry, nephelometry, turbidimetry. UV-VIS spectrophotometry - principle, properties, device design, application.
5. Luminescent methods: molecular fluorescence, phosphorescence, chemiluminescence, bioluminescence and their analytical use.
6. Mass spectrometry.
7. Fundamentals of optical atomic spectrometry.

Topic III: Separation Methods
Basic theory of chromatographic separation process. Planar Chromatography, Column Chromatography.
9. Liquid chromatography
10. Gas chromatography.
11. Separation in the electric field. Planar techniques (gel electrophoresis), capillary techniques (capillary zone electrophoresis, isotachophoresis, capillary isoelectric focusation).
12. Tandem techniques.

Topic IV: Evaluation of results
13. Basics of analytical chemometry. Procedures for evaluating analytical results and calibration functions.

Exercise

26 hod., compulsory

Teacher / Lecturer

doc. Mgr. Michaela Vašinová Galiová, Ph.D.