Course detail

Laboratory Classes in Instrumental Analysis - S2

FCH-MC_IA2_PAcad. year: 2024/2025

The subject aims at practical demostration of analytical techniques which are used in the analysis of materials, biological systems, transport systems for biologically active substances and in technical practice.

Language of instruction

Czech

Number of ECTS credits

Mode of study

Not applicable.

Guarantor

Ing. Jaromír Pořízka, Ph.D.

Department

Institute of Physical and Applied Chemistry (ÚFSCH)

Entry knowledge

Knowledge of general, inorganic, organic, physical, analytical chemistry and biochemistry.

Rules for evaluation and completion of the course

The resulting evaluation is the average of the evaluation of individual protocols.
Mandatory participation in all blaboratory classes.

Aims

The aim of the subject is Improvement of knowledge gained during lectures and familiarize students with methods of instrumental and structural analysis used in the field of chemistry of biologically active substances.
Gaining practical knowledge in the field of analysis of biological samples, selection of the apropriate method, evaluation and interpretation of the results.

Study aids

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Klouda P.: Moderní analytické metody. Nakladatelství Pavel Klouda, Ostrava 2003. (CS)
Králová a kolektiv: Bioanalytické metody. Vysoká škola chemicko-technologická v Praze (CS) (CS)
Študlík a kolektiv: Analytické separační metody. Nakladatelství Karolinum, Praha 2004 (CS) (CS)

Type of course unit

Guided consultation in combined form of studies

26 hod., optionally

Teacher / Lecturer

Ing. Jaromír Pořízka, Ph.D.

Laboratory exercise

39 hod., compulsory

Teacher / Lecturer

doc. Ing. Petr Sedláček, Ph.D.
Ing. Jiří Přibyl
Ing. Jaromír Pořízka, Ph.D.
Ing. Viktorie-Alexandra Pacasová
doc. Ing. Eva Vítová, Ph.D.
Ing. Ludmila Kouřilová
doc. Ing. Filip Mravec, Ph.D.
Ing. Michal Gross
Ing. Zuzana Slavíková, Ph.D.

Syllabus

1. Introduction
2. Liquid chromatography for analysis of medical drugs
3. LC-MS analysis of biological samples
4. Analysis of volatile compounds by gas chromatography
5. UV-VIS spectroscopy as a tool for analysis of enzymes
6. FTIR spectroscopy for analysis of biopolymers
7. Fluorescence spectroscopy as method for analysis of transport of biologically active samples
8. Fluoresecence microscopy analysis at single cell level
9. Gel electrophoresis
10. Determination of fermentation products by CZE
11. Analysis of heavy meatl contamination by ICP-MS
12. Flow cytometry to analysis of cell populations
13. ELISA instrumentace and assays

Elearning

eLearning: currently opened course