Course detail
Chromatography
FCH-MC_CHRAcad. year: 2024/2025
History of chromatography. Basic physical-chemical principles of chromatography. Liquid chromatography - individual techniques based on different separation mechanisms, instrumentation, applications, current trends. Gas chromatography - individual techniques based on different separation mechanisms, instrumentation, applications, current trends. Interpretation of chromatographic data, qualitative and quantitative analysis.
Language of instruction
Number of ECTS credits
Mode of study
Guarantor
Entry knowledge
Rules for evaluation and completion of the course
Attendance at lectures is optional and is not controlled, but it is recommended.
Aims
- Thorough familiarization of students with chromatography as an analytical separation technique
- detailed discussion of chromatographic separation mechanisms, including their capabilities and limitations
- introduction of students to the field of chromatography application in analytical practice
Successful completion of the course the student will gain the following knowledge and skills:
1. Students will be familiarized with the theory of chromatographic separation process and they will be able to apply theoretical knowledge in optimization of chromatographic separation
2. They will be provided with knowledge of instrumentation in liquid, gas and supercritical fluid chromatography
3. They will be able to assess the suitability of chromatographic techniques for solving specific analytical problems and critically evaluate the results.
Study aids
Prerequisites and corequisites
Basic literature
Churáček J. a kol.: Nové trendy v teorii a instrumentaci vybraných analytických metod. SNTL Praha, 1993. (CS)
L. Nováková, M. Douša: Moderní HPLC v teorii a praxi I, 2013 (CS)
L. Nováková, M. Douša: Moderní HPLC v teorii a praxi II, 2013 (CS)
V. R. Meyer: Practical High-Performance. Liquid Chromatography, 2004 (EN)
Recommended reading
Classification of course in study plans
Type of course unit
Lecture
Teacher / Lecturer
Syllabus
2. Basic theory of chromatography – thermodynamical and kinetic aspects, effectivity of chromatographic separation
3. Planar chromatography
4. Column chromatography – basic configuration and theory
5. High performance liquid chromatography – separation principles, instrumentation, columns and rules of their selection, normal and reversed-phase systems, isocratic and gradient elution, ion chromatography, ion-pair chromatography, hydrophobic interaction liquid chromatography, size exclusion chromatography, bioaffinity chromatography, current trends – UPLC, UHPLC
6. Gas chromatography – separation principles, instrumentation, capillary and packed columns, rules for their selection, classification of stationary phases in gas chromatography, derivatization in gas chromatography
7. Tandem techniques in chromatography – systems LC-LC, LC-GC, LC-ICP
8. Tandem techniques in chromatography – systems GC-GC, GCxGC
9. Fast chromatography
10. Qualitative aspects of chromatographic data – correlation structure-retention, retention indices in gas chromatography
11. Quantitation in chromatography
12. Troubleshooting in LC, practical examples
13. Troubleshooting in GC, practical examples
Guided consultation in combined form of studies
Teacher / Lecturer