Course detail

Laboratory Classes in Analytical Chemistry II

FCH-BC_ANC2_PAcad. year: 2024/2025

Practical teaching of selected basic instrumental analytical methods: molecular spectrophotometry and fluorimetry, atomic absorption and emission spectrometry, separation techniques: liquid, gas and thin-layer chromatography, gel electrophoresis and XRF, SEM and NMR methods.

Language of instruction

Czech

Number of ECTS credits

Mode of study

Not applicable.

Guarantor

doc. Mgr. Renata Komendová, Ph.D.

Department

Institute of Chemistry and Technology of Environmental Protection (ÚCHTOŽP)

Entry knowledge

Laboratory course of analytical chemistry I. Practical knowledge of basic laboratory skills: weighing, pipetting, preparation of measuring solutions, titration.
Knowledge of basic calculations used in analytical chemistry, evaluation by the calibration curve method.

Rules for evaluation and completion of the course

Graded course-unit credit
Requirements:
- all laboratory tasks completed, results and reports in good quality
- course test
Final classification is based on average classification of individual tasks.
Analytical results evaluation:
error to
1.5% A
3% B
5% C
7% D
9% E
error over 9% F
Overall classification:
100-90% A
89-80% B
79-70% C
69-60% D
59-50% E
49-0% F
Input e-learning tests to check students' readiness.
Work in analytical laboratory.
Evaluation of analytical results.
Elaboration of the protocols.
Credit test at the end of the course.

Aims

The aim of the Analytical Chemistry Practice II is to teach students the basics and use of selected instrumental analytical methods, such as molecular spectrophotometry and fluorimetry, atomic absorption and emission spectrometry, separation techniques liquid, gas and thin-layer chromatography, gel electrophoresis and XRF, SEM and NMR methods.

Completing the course will increase the students' competences as follows:

1. They will be practically acquainted with selected analytical methods.

2. They will be able to practically apply individual determination procedures.

3. They will practice precise work in the analytical laboratory, preparation of standard solutions and correct principles of working with samples.

4. Students will be able to handle all necessary calculations and will be able to evaluate analytical results obtained by individual analyses.

Study aids

Not applicable.

Prerequisites and corequisites

compulsory prerequisite
Laboratory Classes in Inorganic Chemistry I

Basic literature

Šimek Z., Komendová R., Mašek I., Voznica P. : Kvantitativní analýza. FCH VUT v Brně, Brno 2003. (CS)

Type of course unit

Laboratory exercise

52 hod., compulsory

Teacher / Lecturer

doc. Mgr. Renata Komendová, Ph.D.
Ing. Anna Amrichová
Ing. Veronika Řezáčová, Ph.D.
Ing. Simona Jonášová
Mgr. Jan Richtár, Ph.D.
Ing. Jan Koplík, Ph.D.
Ing. Marie Hamplová
Mgr. Marie Novotná, Ph.D.
Ing. Matěj Březina, Ph.D.
Pavla Kleinová
Mgr. Helena Doležalová Weissmannová, Ph.D.
RNDr. Veronika Ostatná, Ph.D.

Syllabus

1. INTRODUCTION TO PRACTICAL CHEMISTRY, WORK SAFETY, METHODOLOGY OF WORK IN THE ANALYTICAL LABORATORY

2. SPECTROPHOTOMETRY I

3. SPECTROPHOTOMETRY II - MULTICOMPONENT ANALYSIS

4. FLUORIMETRY

5. GEL ELECTROPHORESIS + THIN LAYER CHROMATOGRAPHY

6. FLAME EMISSION SPECTROMETRY

7. LIQUID CHROMATOGRAPHY

8. GAS CHROMATOGRAPHY

9. INFRARED SPECTROMETRY

10. ATOMIC ABSORPTION SPECTROMETRY

11. NMR

12. XRF, SEM

13. CREDIT TEST

Elearning

eLearning: currently opened course