Course detail

Practical Introduction to Nanotechnology

FCH-BC_UN_PAcad. year: 2024/2025

The aim of the course is to familiarize students with basic aspects and techniques utilized for preparation of nanoparticles, nanofibers and nanolayers, and, moreover, also analytical methods employed for analysis of these nanosystems. Students attending the course will gain theoretical knowledge and practical exprerience with preparation of anorganic nanoparticles, liposomes, polyelectrolyte complexes, miceral systems and nanolayers. Furthermore, the course also focuses on analytical methods utilized for nanosystmes characterization such as DLS, SAXS or electron microscopy. Last but not least, attention will be paid also to safety aspects of nanotechnology.

Language of instruction

Czech

Number of ECTS credits

Mode of study

Not applicable.

Guarantor

prof. Ing. Stanislav Obruča, Ph.D.

Department

Institute of Physical and Applied Chemistry (ÚFSCH)

Entry knowledge

basic knowledge on anorganic, organic and physical chemistry and biology

Rules for evaluation and completion of the course

Participation at all experimental exercises, presentation of equivalent reports evaluated by tutors; credit test

Aims

The goal of the course is to familiarize students with techniques utilized for preparation and characterization of nano-systems and nanomaterials for medicine and other advanced applications.
Students attending the course will gain knowledge and practical experience in the field of nano-systems preparation and characterization. The course expand knowledge of the course "Introduction to medical materials" and provides basis for realization of laboratory projects.

Study aids

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

D.S. Goodsell: Bionanotechnology. Wiley 2004 (CS)
Hošek J.: Úvod do nanotechnologie. ČVUT, Praha 2010 (CS)
Ramsden J.: Essentials of Nanotechnology, BookBoon 2009 (CS)

Type of course unit

Laboratory exercise

39 hod., compulsory

Teacher / Lecturer

prof. Ing. Stanislav Obruča, Ph.D.

Syllabus

The course combines theoretical oral lectures and practical laboratory exercises.

1. Introduction, safety aspects of the laboratory part of the course
2. Theroretical lecture - preparation of nanoparticles, polyelectrolyte complexes and naofibers; nanosystems in biotechnology, preparation of liposomes,, microencapsulation, safety aspects of nanotechnology
3. Theroretical lecture concerning analytical methods for characterization of nanoparticles - DLS, analytical centrifugation, XRD, SAXS, fluorescence spectroscopy, rheology, electron microscopy
4. Practical laboratory exercises - preparation of inorganic nanoparticles and their characterization
5. Practical laboratory exercises - deternination of partcile size and zeta-potential by Dynamic Light Scattering (DLS)
6. Practical laboratory exercises - preparation and characterization of liposomes
7. Practical laboratory exercises - aggregation, micellar systems
8. Practical laboratory exercises - preparation of polyelectrolyte complexes
9. Practical laboratory exercises - preparation of nanolayers (spin-coating), characterization of layers by using optical and interference microscopy
10. Practical laboratory exercises - electron microscopy