Course detail

Molecular Biotechnology

FCH-MC_MOBAcad. year: 2023/2024

The course is focused on the explanation of procedures used in molecular biotechnology for construction of genetically modified organisms with required characteristics. It explains the procedures used for gene transfer from one organism to the other with the aim to obtain an useful product including procedures used for optimalisation of gene expression and stability of recombinant proteins in procaryotic and eucaryotic cells. The impact of transgenic microorganisms, plants and animals in our life and their practical use are explained in addition to their construction.

Language of instruction

Czech

Number of ECTS credits

Mode of study

Not applicable.

Guarantor

prof. Mgr. Václav Brázda, Ph.D.

Department

Institute of Food Science and Biotechnology (ÚCHPBT)

Entry knowledge

Pass out the lectures of Molecular genetics I and II is recomended

Rules for evaluation and completion of the course

Examination is written (test) and oral. The student had to proof the understanding of the subject.
Attendance at lectures is not obligatory but it is recommended

Aims

Lectures cover methodologies commonly used in molecular biotechnology in construction of transgenic organims and with the use of these organisms.
Theoretical aspects of various experimental techniques used in molecular biotechnology research in GMO construction and their practical use.

Study aids

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Rosypal S. a kol.: Úvod do molekulární biologie. IV. díl. Vyd. S. Rosypal, Brno, 2006. (CS)

Type of course unit

Lecture

26 hod., optionally

Teacher / Lecturer

prof. Mgr. Václav Brázda, Ph.D.
RNDr. Aleš Kovařík, CSc.
MUDr. Petr Müller, Ph.D.

Syllabus

924/5000
• The relationship between chemical production and biotechnology.
• DNA isolation and recombinant DNA technology.
• DNA cloning, polymerase chain reaction, PCR utilization in clinical and food diagnostics
• Cloning vectors and their use.
• Transformacfe - Transfer of foreign DNA into cells. Selection of cloned gene cells.
• Fusion proteins. Translational expression vectors. Enhancement of protein stability. Integration and shuttle vectors.
• Yeast expression systems. Systems using insect cells, mammalian expression systems.
• Biotechnological use of genetically modified micro-organisms.
• Transgenic plants and animal animals.
• Bioinformatics in gene biotechnology
• Use of bioinformatic approaches in the study of nucleic acids and proteins
• Gene therapy Diseases treatable by gene therapy. Vectors for gene therapy. Perspectives of gene therapy.
• Development, characterization and antibody production

Guided consultation in combined form of studies

26 hod., optionally

Teacher / Lecturer

prof. Mgr. Václav Brázda, Ph.D.

Elearning

eLearning: currently opened course