Course detail

Advanced bioanalytical methods

FCH-DCO_SPZAcad. year: 2022/2023

By disscusions and consultations, students will be familiarized with advanced analytical techniques employed for analysis of biological samples. The focus will be on preparative and analytical separation techniques, advanced spectroscopic techniques, immunochemical methods, molecular techniques, bioinformatics, genetics, transcriptomics, proteomics and metabolomics.

Language of instruction

Czech

Mode of study

Not applicable.

Guarantor

prof. Ing. Stanislav Obruča, Ph.D.

Department

Institute of Food Science and Biotechnology (ÚCHPBT)

Learning outcomes of the course unit

Extending theoretical knowledge of selected procedures used in the analysis of biological systems, in the evaluation of biochemical technological processes and in other sectors working with material of biological nature.

Prerequisites

Without restriction

Co-requisites

Not applicable.

Planned learning activities and teaching methods

The course uses teaching methods in form of individual consultation. The e-learning system (LMS Moodle) is available to teachers and students.

Assesment methods and criteria linked to learning outcomes

colloquium

Course curriculum

Bioinformatic analysis, application of genotypic techniques, polyphasic approach.
Analysis of gene expression.
Analysis of non-culturable microrganisms using metagenomics
Single cell analysisi and manipulation
Advanced spectroscopical techniques for analysis of biological samples
Advanced chromatography and electrophoresis for analysis of biological materials
Preparation, isolation, pufrification and characterization of proteins.
Utilization of advanced fluorescent techniques for analysis of biological samples
Analytical imunochemistry
Genomics, transcriptomics, proteomics, metabolomics

Work placements

Not applicable.

Aims

Lectures proceed methods for food products processing

Specification of controlled education, way of implementation and compensation for absences

In form of personal consultations and discussions

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Králová B. : Bioanalytické metody. Vysoká škola chemicko-technologická v Praze, Praha 2001. (CS)
Manz A., Dittrich P.S., Pamme N., Iossifidis D.: Bioanalytical Chemistry. Imperial College Press, 2015. 256 s. (CS)