Course detail

Laboratory Classes in Instrumental Analysis - S2

FCH-MC_IA2_PAcad. year: 2022/2023

The subject aims at practical demostration of analytical techniques which are used in the analysis of materials, biological systems, transport systems for biologically active substances and in technical practice.

Language of instruction

Czech

Number of ECTS credits

Mode of study

Not applicable.

Guarantor

Ing. Jaromír Pořízka, Ph.D.

Department

Institute of Physical and Applied Chemistry (ÚFSCH)

Learning outcomes of the course unit

Gaining practical knowledge in the field of analysis of biological samples, selection of the apropriate method, evaluation and interpretation of the results.

Prerequisites

Knowledge of general, inorganic, organic, physical, analytical chemistry and biochemistry.

Co-requisites

Not applicable.

Planned learning activities and teaching methods

The course uses teaching methods in form of Laboratory exercise - 3 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.

Assesment methods and criteria linked to learning outcomes

The resulting evaluation is the average of the evaluation of individual protocols.

Course curriculum

1. Introduction
2. Liquid chromatography for analysis of medical drugs
3. LC-MS analysis of biological samples
4. Analysis of volatile compounds by gas chromatography
5. UV-VIS spectroscopy as a tool for analysis of enzymes
6. FTIR spectroscopy for analysis of biopolymers
7. Fluorescence spectroscopy as method for analysis of transport of biologically active samples
8. Fluoresecence microscopy analysis at single cell level
9. Gel electrophoresis
10. Determination of fermentation products by CZE
11. Analysis of heavy meatl contamination by ICP-MS
12. Flow cytometry to analysis of cell populations
13. ELISA instrumentace and assays

Work placements

Not applicable.

Aims

The aim of the subject is Improvement of knowledge gained during lectures and familiarize students with methods of instrumental and structural analysis used in the field of chemistry of biologically active substances.

Specification of controlled education, way of implementation and compensation for absences

Mandatory participation in all blaboratory classes.

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Klouda P.: Moderní analytické metody. Nakladatelství Pavel Klouda, Ostrava 2003. (CS)
Králová a kolektiv: Bioanalytické metody. Vysoká škola chemicko-technologická v Praze (CS) (CS)
Študlík a kolektiv: Analytické separační metody. Nakladatelství Karolinum, Praha 2004 (CS) (CS)

Type of course unit

Guided consultation in combined form of studies

26 hod., optionally

Teacher / Lecturer

Ing. Jaromír Pořízka, Ph.D.

Laboratory exercise

39 hod., compulsory

Teacher / Lecturer

Ing. Eva Slaninová, Ph.D.
Ing. Michal Gross
Ing. Jitka Krouská, Ph.D.
doc. Ing. Petr Sedláček, Ph.D.
doc. Ing. Filip Mravec, Ph.D.
Ing. Zuzana Juglová
Ing. Jaromír Pořízka, Ph.D.
Ing. Martina Havlíková, Ph.D.
doc. RNDr. Renata Mikulíková, Ph.D.
Ing. Diana Černayová
Ing. Štěpánka Trachtová, Ph.D.
Ing. Martin Gajdušek

Elearning

eLearning: currently opened course