Course detail
Laboratory Classes in Instrumental and Structure Analysis - MA
FCH-MCA_ISA_CHMAAcad. year: 2022/2023
The subject aims at practical demostration of analytical techniques which are used in the analysis of materials, biological systems, transport systems for biologically active substances and in technical practice.
Language of instruction
Number of ECTS credits
Mode of study
Guarantor
Learning outcomes of the course unit
Prerequisites
Co-requisites
Planned learning activities and teaching methods
Assesment methods and criteria linked to learning outcomes
Course curriculum
2. Liquid chromatography for analysis of medical drugs
3. LC-MS analysis of biological samples
4. Analysis of volatile compounds by gas chromatography
5. UV-VIS spectroscopy as a tool for analysis of enzymes
6. FTIR spectroscopy for analysis of biopolymers
7. Fluorescence spectroscopy as method for analysis of transport of biologically active samples
8. Fluoresecence microscopy analysis at single cell level
9. Gel electrophoresis
10. Determination of fermentation products by CZE
11. Analysis of heavy meatl contamination by ICP-MS
12. Flow cytometry to analysis of cell populations
13. Use of DLS to determine stability and other properties of proteins
Work placements
Aims
- improve knowledge gained during lectures
- familiarize students with methods of instrumental and structural analysis used in the field of medical applications
Specification of controlled education, way of implementation and compensation for absences
Recommended optional programme components
Prerequisites and corequisites
Basic literature
Králová a kolektiv: Bioanalytické metody. Vysoká škola chemicko-technologická v Praze, Praha 2001. (CS)
Študlík a kolektiv: Analytické separační metody. Nakladatelství Karolinum, Praha 2004 (CS)
Recommended reading
Classification of course in study plans
Type of course unit
Guided consultation in combined form of studies
Teacher / Lecturer