Course detail
Mass Spectrometry
FCH-MC_HMSAcad. year: 2022/2023
Physical bases of mass spectrometry, MS history. Ionization techniques - overview, development, description of contemporary used techniquies (electron ionization, chemical ionization, electrospray, chemical ionization at atmospheric pressure etc). Analyzers in mass spectrometry (static - magnetic, electrostatic, quadrupole, 3D- and linear ion trap, Time-of-Flight, ion cyclotron resonance, orbitrap). Detectors and vacuum systems. Hyphenated and tandem techniquies. Interpretation of mass spectra with respect to the ionization technique applied.
Language of instruction
Number of ECTS credits
Mode of study
Guarantor
Learning outcomes of the course unit
1. Students will be familiar with the principles of functions of various types of mass spectrometers;
2. Students will be able to choose the optimal device for highly efficient solutions for specific analytical problems;
3. Students will know the basics of mass spectra interpretation, depending on the ionization technique used and the analyzer resolution.
Prerequisites
Good knowledge of basic, inorganic, organic, physical and analytical chemistry
Co-requisites
Planned learning activities and teaching methods
Assesment methods and criteria linked to learning outcomes
Course curriculum
2. Instrumentation of mass spektrometry.
3. Ionization techniques in mass spektrometry.
4. Mass analyzers.
5. Hyphenated techniques (GC/MS, LC/MS, CE/MS).
6. Hyphenated techniques (MS/MS).
7. Mass spectrometry imaging.
8. Inorganic mass spectrometry.
9. Interpretation of mass spectra using electron ionization, fragmentation rules.
10. Interpretation of mass spectra – practicing.
11. Interpretation of mass spectra using soft ionization techniques.
12. Interpretation of mass spectra using soft ionization techniques – practicing.
13. Practical applications of mass spectrometry.
Work placements
Aims
- to familiarize students with the theoretical foundations of mass spectrometry
- to provide an overview of current instrumentation of mass spectrometry
- to introduce students to the basic concepts interpretation of mass spectra
Specification of controlled education, way of implementation and compensation for absences
Recommended optional programme components
Prerequisites and corequisites
Basic literature
J. H. Gross: Mass Spectrometry, Third Edition, 2017 (EN)
K. Záruba a spol.: Analytická chemie 2. díl, 2016 (CS)
Recommended reading
Elearning
Classification of course in study plans
- Programme NKCP_ECHT Master's 2 year of study, winter semester, compulsory-optional
- Programme NKCP_CHTOZP Master's
branch NKCO_CHTOZP , 2 year of study, winter semester, compulsory-optional
- Programme NPCP_CHTOZP Master's
branch NPCO_CHTOZP , 2 year of study, winter semester, compulsory-optional
- Programme NPCP_ECHT Master's 2 year of study, winter semester, compulsory-optional
Type of course unit
Guided consultation in combined form of studies
Teacher / Lecturer
Elearning