Course detail
Laboratory Classes in Instrumental and Structure Analysis - FSBT
FCH-MCA_ISA_PAcad. year: 2021/2022
Practical class to the lecture Instrumental and Structure Analysis.
Students are familiarized with instrumental analytical techniques used by control and analysis of natural substances during this class. The course practically develops knowledge from the subjects Analytical Chemistry II and Instrumental and Structural Analysis. Individual tasks and laboratory equipment were chosen so that students come into direct contact with modern infrastructure in solving specific problems of analysis of samples of natural substances. Practical exercises are focused on separation techniques (chromatography and electrophoresis), elemental analysis, spectral and structural analysis.
Language of instruction
Number of ECTS credits
Mode of study
Guarantor
Learning outcomes of the course unit
Prerequisites
Co-requisites
Planned learning activities and teaching methods
Teaching in the distance form is performed in the block form on the basis of individual agreement between teachers and students.
Assesment methods and criteria linked to learning outcomes
Course curriculum
1. Optical emmision spectrometry with inductively coupled plasma - ICP-OES
2. Mass spectrometry with inductively coupled plasma - ICP-MS
3. Electromigration methods - CZE
4. Electromigration methods - chip gel electrophoresis
5. Liquid chromatography - HPLC
6. Liquid chromatography with mass detection - LC-MS
7. Gas chromatography - GC
8. Gas chromatography with mass detection - GC-MS
9. UV-VIS Spectrophotometry - UV-VIS
10. Infrared spectroscopy – IR
Work placements
Aims
Specification of controlled education, way of implementation and compensation for absences
The examination of basic knowledge relating to the particular project is done at the beginning of each exercise. Each student will write out laboratory diary. Results from the practise will be summarized as hand-writen laboratory protocol, which will be part of laboratory diary.
Laboratory reports are required till 7 days after the last exercise. Classification of the student will be based on evaluation of laboratory diary, protocols and activity in laboratory.
Recommended optional programme components
Prerequisites and corequisites
Basic literature
Mikuš P., Piešťanský J., Dokupilová S.: Kvapalinová chromatografia, hmotnosná spektrometria, Veda, Bratislava, Slovensko, 2018. (SK)
Milata V.: Vybrané metódy molekulovej spektroskopie, STU, Bratislava, Slovensko, 2007. (SK)
Nováková L., Douša M. a kol.: Moderní HPLC separace v teorii a praxi I, Europrint, Praha, Česká republika, 2013. (CS)
Nováková L., Douša M. a kol.: Moderní HPLC separace v teorii a praxi II, Europrint, Praha, Česká republika, 2013. (CS)
Skoog D., West D.M., Holler F.J., Crouch S.R.: Analytická chemie, ČVUT, Praha, Česká republika, 2019. (CS)
Recommended reading
Classification of course in study plans
Type of course unit
Guided consultation in combined form of studies
Teacher / Lecturer