Course detail
Bioanalytical Methods
FCH-MC_BIMAcad. year: 2021/2022
Not applicable.
Language of instruction
Number of ECTS credits
Mode of study
Guarantor
Learning outcomes of the course unit
Prerequisites
Co-requisites
Planned learning activities and teaching methods
Assesment methods and criteria linked to learning outcomes
Course curriculum
2) Overview of bioanalytical techniques, basic principles of biopolymer analysis
3) Enzymology - Enzyme distribution, enzyme kinetics, enzymes as analytical reagents
4) Enzymology - immobilization of enzymes, use in practice
5) Immunochemical Methods - Principles and Overview of Antibody Techniques
6) Immunochemical methods - fluorescence, bioluminescence, radioactive and enzyme immunoassay
7) Nanotechnology, biosensors, construction and applications
8) Chromatographic techniques - gel permeation, affinity and ion exchange chromatography, gas (GC) and liquid chromatography (HPLC, UHPLC), supercritical fluid chromatography (SFC) and their use in biomolecular analysis
9) Spectrophotometric and microscopic methods - optical, fluorescence, infrared, raman and high-frequency spectroscopy, nuclear magnetic resonance, atomic absorption and emission spectrometry
10) Electrophoretic methods - electrophoresis, isoelectric focusing, isotachophoresis, affinity, two-dimensional, zonal and capillary electrophoresis.
11) Molecular techniques, polymerase chain reaction (PCR), RT-PCR detection of specific product sequences in situ, DNA and RNA chips, examples of practical use
12) Biological and microbiological analytical methods, flow cytometry, fluorescence techniques, electron microscopy
13) Modern bioanalytical techniques
Work placements
Aims
Specification of controlled education, way of implementation and compensation for absences
Recommended optional programme components
Prerequisites and corequisites
Basic literature
Záruba, Kamil. Analytická chemie. Praha: VSCHT v Praze, 2016. (CS)
Recommended reading
UGO, Paolo and Coll. Bioanalytical Chemistry. De Gruyter, 2021. (EN)
Elearning
Classification of course in study plans
- Programme NPCP_CHMA Master's
specialization BF , 2 year of study, winter semester, compulsory-optional
specialization CHBL , 2 year of study, winter semester, compulsory-optional - Programme NPCP_CHTPO Master's
specialization PCH , 2 year of study, winter semester, compulsory-optional
specialization CHPL , 2 year of study, winter semester, compulsory-optional - Programme NKCP_CHTPO Master's
specialization PCH , 2 year of study, winter semester, compulsory-optional
specialization CHPL , 2 year of study, winter semester, compulsory-optional
specialization BCH , 2 year of study, winter semester, compulsory-optional - Programme NPCP_CHTP Master's
branch NPCO_CHTP , 2 year of study, winter semester, compulsory-optional
- Programme NKCP_CHTP Master's
branch NKCO_CHTP , 2 year of study, winter semester, compulsory-optional
- Programme NPCP_CHPL Master's 2 year of study, winter semester, compulsory-optional
- Programme NKCP_CHPL Master's 2 year of study, winter semester, compulsory-optional
Type of course unit
Guided consultation in combined form of studies
Teacher / Lecturer
Elearning