Czech

Not applicable.

Gaining practical knowledge in the field of analysis of biological samples, selection of the apropriate method, evaluation and interpretation of the results.

Knowledge of general, inorganic, organic, physical, analytical chemistry and biochemistry.

Not applicable.

The course uses teaching methods in form of Laboratory exercise - 3 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.

The resulting evaluation is the average of the evaluation of individual protocols.

1. Introduction
2. Liquid chromatography for analysis of medical drugs
3. LC-MS analysis of biological samples
4. Analysis of volatile compounds by gas chromatography
5. UV-VIS spectroscopy as a tool for analysis of enzymes
6. FTIR spectroscopy for analysis of biopolymers
7. Fluorescence spectroscopy as method for analysis of transport of biologically active samples
8. Fluoresecence microscopy analysis at single cell level
9. Gel electrophoresis
10. Determination of fermentation products by CZE
11. Analysis of heavy meatl contamination by ICP-MS
12. Flow cytometry to analysis of cell populations
13. ELISA instrumentace and assays

Not applicable.

The aim of the subject is Improvement of knowledge gained during lectures and familiarize students with methods of instrumental and structural analysis used in the field of chemistry of biologically active substances.

Mandatory participation in all blaboratory classes.

Not applicable.

Not applicable.

Klouda P.: Moderní analytické metody. Nakladatelství Pavel Klouda, Ostrava 2003. (CS)
Králová a kolektiv: Bioanalytické metody. Vysoká škola chemicko-technologická v Praze (CS) (CS)
Študlík a kolektiv: Analytické separační metody. Nakladatelství Karolinum, Praha 2004 (CS) (CS)

Not applicable.